PlantSME Plant secondary metabolism enzyme database
Rosmarinic acid portable pathway

Rosmarinic acid portable pathway

Category：Rosmarinic acid pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route
Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.
7 curated steps/modules
Phenylalanine Donor Branch
01
Substrate
L-Phenylalanine
C9H11NO2
C6H5-CH2-CH(NH2)-COOH
Deamination into the phenylpropanoid donor branch
PAL34
Product
trans-Cinnamic acid
C9H8O2
C6H5-CH=CH-COOH
PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.
02
Substrate
trans-Cinnamic acid
C9H8O2
C6H5-CH=CH-COOH
Cinnamate 4-hydroxylation
C4H271
Product
4-Coumaric acid
C9H8O3
4-HO-C6H4-CH=CH-COOH
C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.
03
Substrate
4-Coumaric acid
C9H8O3
4-HO-C6H4-CH=CH-COOH
Substrate
CoA
C21H36N7O16P3S
CoA-SH
CoA ligation to form the RAS acyl donor
4CL122
Product
4-Coumaroyl-CoA
C30H38N7O18P3S
4-HO-C6H4-CH=CH-C(=O)-SCoA
4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.
Tyrosine Acceptor Branch And Condensation
04
Substrate
L-Tyrosine
C9H11NO3
4-HO-C6H4-CH2-CH(NH2)-COOH
Transamination to 4-hydroxyphenylpyruvate
TAT30
Product
4-Hydroxyphenylpyruvate
C9H8O4
4-HO-C6H4-CH2-C(=O)-COOH
TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.
05
Substrate
4-Hydroxyphenylpyruvate
C9H8O4
4-HO-C6H4-CH2-C(=O)-COOH
Reduction to the HPLA acceptor
HPPR45
Product
4-Hydroxyphenyllactate
C9H10O4
4-HO-C6H4-CH2-CH(OH)-COOH
HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.
06
Substrate
4-Coumaroyl-CoA
C30H38N7O18P3S
4-HO-C6H4-CH=CH-C(=O)-SCoA
Substrate
4-Hydroxyphenyllactate
C9H10O4
4-HO-C6H4-CH2-CH(OH)-COOH
Ester-forming condensation
RAS114
Product
4-Coumaroyl-4'-hydroxyphenyllactate
C18H16O7
4-HO-C6H4-CH=CH-C(=O)-O-CH(COOH)-CH2-Ar-OH
RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.
07
Substrate
4-Coumaroyl-4'-hydroxyphenyllactate
C18H16O7
mono-catechol ester precursor
CYP98A14-type hydroxylation with CPR electron-transfer support
CPR48
Product
Rosmarinic acid
C18H16O8
caffeoyl-O-3,4-dihydroxyphenyllactate
CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.
Lineage Distribution
Assignments under this module are distributed across the following plant lineages.
All Lineages Lycophytes/Ferns (664)
Current lineage filter: Lycophytes/Ferns. All species, family, module, and gene statistics below have been narrowed to this lineage.
Algae
Hits0
Bryophytes
Hits0
Lycophytes/Ferns
Hits664
Gymnosperms
Hits0
Basal angiosperms
Hits0
Monocots
Hits0
Eudicots
Hits0
Species Distribution
Azolla filiculoides
Isoetes taiwanensis
Selaginella moellendorffii
Salvinia cucullata
Lineage Summary
Lycophytes/Ferns
664
Module Gene List
Reset
GeneSpeciesProtein ID
ItaiItaiw_v1_scaffold_90399000.1 isoetes_taiwanensis ItaiItaiw_v1_scaffold_90399000.1
ItaiItaiw_v1_scaffold_930405850.1 isoetes_taiwanensis ItaiItaiw_v1_scaffold_930405850.1
ItaiItaiw_v1_scaffold_940407330.1 isoetes_taiwanensis ItaiItaiw_v1_scaffold_940407330.1
ScucSacu_v1.1_s00010000260 salvinia_cucullata ScucSacu_v1.1_s00010000260
ScucSacu_v1.1_s00010001530 salvinia_cucullata ScucSacu_v1.1_s00010001530
ScucSacu_v1.1_s00030011320 salvinia_cucullata ScucSacu_v1.1_s00030011320
ScucSacu_v1.1_s00040012150 salvinia_cucullata ScucSacu_v1.1_s00040012150
ScucSacu_v1.1_s00100027560 salvinia_cucullata ScucSacu_v1.1_s00100027560
ScucSacu_v1.1_s00150040780 salvinia_cucullata ScucSacu_v1.1_s00150040780
ScucSacu_v1.1_s00150041160 salvinia_cucullata ScucSacu_v1.1_s00150041160
ScucSacu_v1.1_s00160042260 salvinia_cucullata ScucSacu_v1.1_s00160042260
ScucSacu_v1.1_s00170044610 salvinia_cucullata ScucSacu_v1.1_s00170044610
ScucSacu_v1.1_s00190050410 salvinia_cucullata ScucSacu_v1.1_s00190050410
ScucSacu_v1.1_s00260061710 salvinia_cucullata ScucSacu_v1.1_s00260061710
ScucSacu_v1.1_s00270062690 salvinia_cucullata ScucSacu_v1.1_s00270062690
ScucSacu_v1.1_s00310068900 salvinia_cucullata ScucSacu_v1.1_s00310068900
ScucSacu_v1.1_s00320070440 salvinia_cucullata ScucSacu_v1.1_s00320070440
ScucSacu_v1.1_s00390080240 salvinia_cucullata ScucSacu_v1.1_s00390080240
ScucSacu_v1.1_s00400081050 salvinia_cucullata ScucSacu_v1.1_s00400081050
ScucSacu_v1.1_s00550096900 salvinia_cucullata ScucSacu_v1.1_s00550096900
ScucSacu_v1.1_s00610103840 salvinia_cucullata ScucSacu_v1.1_s00610103840
ScucSacu_v1.1_s00660109280 salvinia_cucullata ScucSacu_v1.1_s00660109280
ScucSacu_v1.1_s00670109800 salvinia_cucullata ScucSacu_v1.1_s00670109800
ScucSacu_v1.1_s00910128240 salvinia_cucullata ScucSacu_v1.1_s00910128240
ScucSacu_v1.1_s00930130600 salvinia_cucullata ScucSacu_v1.1_s00930130600
ScucSacu_v1.1_s01150144720 salvinia_cucullata ScucSacu_v1.1_s01150144720
ScucSacu_v1.1_s01180146180 salvinia_cucullata ScucSacu_v1.1_s01180146180
ScucSacu_v1.1_s01250149600 salvinia_cucullata ScucSacu_v1.1_s01250149600
ScucSacu_v1.1_s01260150000 salvinia_cucullata ScucSacu_v1.1_s01260150000
ScucSacu_v1.1_s01270150660 salvinia_cucullata ScucSacu_v1.1_s01270150660