PlantSME Plant secondary metabolism enzyme database
Rosmarinic acid portable pathway

Rosmarinic acid portable pathway

Category：Rosmarinic acid pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route
Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.
7 curated steps/modules
Phenylalanine Donor Branch
01
Substrate
L-Phenylalanine
C9H11NO2
C6H5-CH2-CH(NH2)-COOH
Deamination into the phenylpropanoid donor branch
PAL7015
Product
trans-Cinnamic acid
C9H8O2
C6H5-CH=CH-COOH
PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.
02
Substrate
trans-Cinnamic acid
C9H8O2
C6H5-CH=CH-COOH
Cinnamate 4-hydroxylation
C4H48992
Product
4-Coumaric acid
C9H8O3
4-HO-C6H4-CH=CH-COOH
C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.
03
Substrate
4-Coumaric acid
C9H8O3
4-HO-C6H4-CH=CH-COOH
Substrate
CoA
C21H36N7O16P3S
CoA-SH
CoA ligation to form the RAS acyl donor
4CL25914
Product
4-Coumaroyl-CoA
C30H38N7O18P3S
4-HO-C6H4-CH=CH-C(=O)-SCoA
4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.
Tyrosine Acceptor Branch And Condensation
04
Substrate
L-Tyrosine
C9H11NO3
4-HO-C6H4-CH2-CH(NH2)-COOH
Transamination to 4-hydroxyphenylpyruvate
TAT14219
Product
4-Hydroxyphenylpyruvate
C9H8O4
4-HO-C6H4-CH2-C(=O)-COOH
TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.
05
Substrate
4-Hydroxyphenylpyruvate
C9H8O4
4-HO-C6H4-CH2-C(=O)-COOH
Reduction to the HPLA acceptor
HPPR5065
Product
4-Hydroxyphenyllactate
C9H10O4
4-HO-C6H4-CH2-CH(OH)-COOH
HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.
06
Substrate
4-Coumaroyl-CoA
C30H38N7O18P3S
4-HO-C6H4-CH=CH-C(=O)-SCoA
Substrate
4-Hydroxyphenyllactate
C9H10O4
4-HO-C6H4-CH2-CH(OH)-COOH
Ester-forming condensation
RAS13433
Product
4-Coumaroyl-4'-hydroxyphenyllactate
C18H16O7
4-HO-C6H4-CH=CH-C(=O)-O-CH(COOH)-CH2-Ar-OH
RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.
07
Substrate
4-Coumaroyl-4'-hydroxyphenyllactate
C18H16O7
mono-catechol ester precursor
CYP98A14-type hydroxylation with CPR electron-transfer support
CPR5870
Product
Rosmarinic acid
C18H16O8
caffeoyl-O-3,4-dihydroxyphenyllactate
CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.
Lineage Distribution
Assignments under this module are distributed across the following plant lineages.
All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)
Algae
Hits388
Bryophytes
Hits9418
Lycophytes/Ferns
Hits423
Gymnosperms
Hits1285
Basal angiosperms
Hits2118
Monocots
Hits23710
Eudicots
Hits83094
Species Distribution
Triticum turgidum
Aegilops tauschii
Hordeum vulgare
Triticum aestivum
Prunus domestica
Ocimum basilicum
Avena sativa
Papaver setigerum
Pogostemon cablin
Fragaria ananassa
Lineage Summary
Algae
388
Bryophytes
9418
Lycophytes/Ferns
423
Gymnosperms
1285
Basal angiosperms
2118
Monocots
23710
Eudicots
83094
Module Gene List
GeneSpeciesProtein ID
acorus_gramineus_AGRA_00692 acorus_gramineus acorus_gramineus_AGRA_00692
acorus_gramineus_AGRA_00783 acorus_gramineus acorus_gramineus_AGRA_00783
acorus_gramineus_AGRA_00880 acorus_gramineus acorus_gramineus_AGRA_00880
acorus_gramineus_AGRA_00920 acorus_gramineus acorus_gramineus_AGRA_00920
acorus_gramineus_AGRA_00998 acorus_gramineus acorus_gramineus_AGRA_00998
acorus_gramineus_AGRA_01122 acorus_gramineus acorus_gramineus_AGRA_01122
acorus_gramineus_AGRA_01171 acorus_gramineus acorus_gramineus_AGRA_01171
acorus_gramineus_AGRA_01272 acorus_gramineus acorus_gramineus_AGRA_01272
acorus_gramineus_AGRA_01296 acorus_gramineus acorus_gramineus_AGRA_01296
acorus_gramineus_AGRA_01398 acorus_gramineus acorus_gramineus_AGRA_01398
acorus_gramineus_AGRA_01456 acorus_gramineus acorus_gramineus_AGRA_01456
acorus_gramineus_AGRA_01782 acorus_gramineus acorus_gramineus_AGRA_01782
acorus_gramineus_AGRA_01939 acorus_gramineus acorus_gramineus_AGRA_01939
acorus_gramineus_AGRA_01940 acorus_gramineus acorus_gramineus_AGRA_01940
acorus_gramineus_AGRA_02298 acorus_gramineus acorus_gramineus_AGRA_02298
acorus_gramineus_AGRA_02299 acorus_gramineus acorus_gramineus_AGRA_02299
acorus_gramineus_AGRA_02300 acorus_gramineus acorus_gramineus_AGRA_02300
acorus_gramineus_AGRA_02649 acorus_gramineus acorus_gramineus_AGRA_02649
acorus_gramineus_AGRA_02685 acorus_gramineus acorus_gramineus_AGRA_02685
acorus_gramineus_AGRA_02686 acorus_gramineus acorus_gramineus_AGRA_02686
acorus_gramineus_AGRA_02735 acorus_gramineus acorus_gramineus_AGRA_02735
acorus_gramineus_AGRA_02859 acorus_gramineus acorus_gramineus_AGRA_02859
acorus_gramineus_AGRA_02861 acorus_gramineus acorus_gramineus_AGRA_02861
acorus_gramineus_AGRA_02947 acorus_gramineus acorus_gramineus_AGRA_02947
acorus_gramineus_AGRA_02948 acorus_gramineus acorus_gramineus_AGRA_02948
acorus_gramineus_AGRA_02990 acorus_gramineus acorus_gramineus_AGRA_02990
acorus_gramineus_AGRA_02992 acorus_gramineus acorus_gramineus_AGRA_02992
acorus_gramineus_AGRA_03003 acorus_gramineus acorus_gramineus_AGRA_03003
acorus_gramineus_AGRA_03004 acorus_gramineus acorus_gramineus_AGRA_03004
acorus_gramineus_AGRA_03138 acorus_gramineus acorus_gramineus_AGRA_03138