Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT13441 SQE4023 CAS46700

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT324927 SMO1/SMO26895 CPI1777 CYP51G14290 FK858 HYD1962 DWF76507 DWF5271 DWF11016

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET22338 DWF4/CYP90B12383 CPD/CYP90A12379 ROT3/CYP90C1396 CYP90D167 BR6OX1/CYP85A1120 BR6OX2/CYP85A265

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Eudicots (108415)

Current lineage filter: Eudicots. All species, family, module, and gene statistics below have been narrowed to this lineage.

Species Distribution

Chrysanthemum seticuspe

Dianthus caryophyllus

Lineage Summary

Module Gene List

Gene	Species	Protein ID
Agluscaffold11144_cov1030020640	alnus_glutinosa	Agluscaffold11144_cov1030020640
Agluscaffold1126_cov630022410	alnus_glutinosa	Agluscaffold1126_cov630022410
Agluscaffold11361_cov1080023500	alnus_glutinosa	Agluscaffold11361_cov1080023500
Agluscaffold11470_cov770024410	alnus_glutinosa	Agluscaffold11470_cov770024410
Agluscaffold11611_cov920026000	alnus_glutinosa	Agluscaffold11611_cov920026000
Agluscaffold11793_cov930030670	alnus_glutinosa	Agluscaffold11793_cov930030670
Agluscaffold1193_cov960031640	alnus_glutinosa	Agluscaffold1193_cov960031640
Agluscaffold11967_cov980031680	alnus_glutinosa	Agluscaffold11967_cov980031680
Agluscaffold126020_cov510040020	alnus_glutinosa	Agluscaffold126020_cov510040020
Agluscaffold12940_cov580044030	alnus_glutinosa	Agluscaffold12940_cov580044030
Agluscaffold1312_cov740047020	alnus_glutinosa	Agluscaffold1312_cov740047020
Agluscaffold1312_cov740047040	alnus_glutinosa	Agluscaffold1312_cov740047040
Agluscaffold132814_cov950048740	alnus_glutinosa	Agluscaffold132814_cov950048740
Agluscaffold132814_cov950048760	alnus_glutinosa	Agluscaffold132814_cov950048760
Agluscaffold13641_cov890054690	alnus_glutinosa	Agluscaffold13641_cov890054690
Agluscaffold13641_cov890054710	alnus_glutinosa	Agluscaffold13641_cov890054710
Agluscaffold14870_cov890068310	alnus_glutinosa	Agluscaffold14870_cov890068310
Agluscaffold15556_cov1080074440	alnus_glutinosa	Agluscaffold15556_cov1080074440
Agluscaffold15717_cov1000076240	alnus_glutinosa	Agluscaffold15717_cov1000076240
Agluscaffold15717_cov1000076250	alnus_glutinosa	Agluscaffold15717_cov1000076250
Agluscaffold15718_cov900076260	alnus_glutinosa	Agluscaffold15718_cov900076260
Agluscaffold15922_cov1020078880	alnus_glutinosa	Agluscaffold15922_cov1020078880
Agluscaffold16020_cov1000079770	alnus_glutinosa	Agluscaffold16020_cov1000079770
Agluscaffold16388_cov840084400	alnus_glutinosa	Agluscaffold16388_cov840084400
Agluscaffold16455_cov990084990	alnus_glutinosa	Agluscaffold16455_cov990084990
Agluscaffold17508_cov900093710	alnus_glutinosa	Agluscaffold17508_cov900093710
Agluscaffold17508_cov900093730	alnus_glutinosa	Agluscaffold17508_cov900093730
Agluscaffold1762_cov1040095730	alnus_glutinosa	Agluscaffold1762_cov1040095730
Agluscaffold17928_cov1030098090	alnus_glutinosa	Agluscaffold17928_cov1030098090
Agluscaffold185545_cov1050103580	alnus_glutinosa	Agluscaffold185545_cov1050103580