Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT14389 SQE4569 CAS35857

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT327781 SMO1/SMO24067 CPI11261 CYP51G1190 FK1234 HYD11364 DWF71524 DWF5888 DWF11689

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET21316 DWF4/CYP90B1290 CPD/CYP90A1103 ROT3/CYP90C1193 CYP90D1142 BR6OX1/CYP85A140 BR6OX2/CYP85A268

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (569) Bryophytes (5698) Lycophytes/Ferns (189) Gymnosperms (791) Basal angiosperms (1411) Monocots (17019) Eudicots (61245)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
Aalbaalba5_s000110130075480.1	abies_alba	Aalbaalba5_s000110130075480.1
Aalbaalba5_s000142680092570.1	abies_alba	Aalbaalba5_s000142680092570.1
Aalbaalba5_s000223900132690.1	abies_alba	Aalbaalba5_s000223900132690.1
Aalbaalba5_s000296170164300.1	abies_alba	Aalbaalba5_s000296170164300.1
Aalbaalba5_s000458080234050.1	abies_alba	Aalbaalba5_s000458080234050.1
Aalbaalba5_s000465870237190.1	abies_alba	Aalbaalba5_s000465870237190.1
Aalbaalba5_s000547350267900.1	abies_alba	Aalbaalba5_s000547350267900.1
Aalbaalba5_s000743420331630.1	abies_alba	Aalbaalba5_s000743420331630.1
Aalbaalba5_s000955410391760.1	abies_alba	Aalbaalba5_s000955410391760.1
Aalbaalba5_s001208230454170.1	abies_alba	Aalbaalba5_s001208230454170.1
Aalbaalba5_s001256290465070.1	abies_alba	Aalbaalba5_s001256290465070.1
Aalbaalba5_s001286920471740.1	abies_alba	Aalbaalba5_s001286920471740.1
Aalbaalba5_s001343110483510.1	abies_alba	Aalbaalba5_s001343110483510.1
Aalbaalba5_s001346820484230.1	abies_alba	Aalbaalba5_s001346820484230.1
Aalbaalba5_s001447540504960.1	abies_alba	Aalbaalba5_s001447540504960.1
Aalbaalba5_s002541080670410.1	abies_alba	Aalbaalba5_s002541080670410.1
Aalbaalba5_s002607460677910.1	abies_alba	Aalbaalba5_s002607460677910.1
Aalbaalba5_s003009800716090.1	abies_alba	Aalbaalba5_s003009800716090.1
Aalbaalba5_s003140240726900.1	abies_alba	Aalbaalba5_s003140240726900.1
Aalbaalba5_s004119220789520.1	abies_alba	Aalbaalba5_s004119220789520.1
Aalbaalba5_s004122830789840.1	abies_alba	Aalbaalba5_s004122830789840.1
Aalbaalba5_s004229920795560.1	abies_alba	Aalbaalba5_s004229920795560.1
Aalbaalba5_s004314320799690.1	abies_alba	Aalbaalba5_s004314320799690.1
Aalbaalba5_s006642630873240.1	abies_alba	Aalbaalba5_s006642630873240.1
Aalbaalba5_s008270780896720.1	abies_alba	Aalbaalba5_s008270780896720.1
Aalbaalba5_s008482770898910.1	abies_alba	Aalbaalba5_s008482770898910.1
Aalbaalba5_s009540710908630.1	abies_alba	Aalbaalba5_s009540710908630.1
Aalbaalba5_s012503700925880.1	abies_alba	Aalbaalba5_s012503700925880.1
XP_027333311.1	abrus_precatorius	XP_027333311.1
XP_027335147.1	abrus_precatorius	XP_027335147.1