Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_hemsleyana_GWHPCBQA000153	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA000153
actinidia_hemsleyana_GWHPCBQA000154	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA000154
actinidia_hemsleyana_GWHPCBQA000173	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA000173
actinidia_hemsleyana_GWHPCBQA000221	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA000221
actinidia_hemsleyana_GWHPCBQA000486	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA000486
actinidia_hemsleyana_GWHPCBQA001010	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001010
actinidia_hemsleyana_GWHPCBQA001088	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001088
actinidia_hemsleyana_GWHPCBQA001130	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001130
actinidia_hemsleyana_GWHPCBQA001142	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001142
actinidia_hemsleyana_GWHPCBQA001254	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001254
actinidia_hemsleyana_GWHPCBQA001255	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001255
actinidia_hemsleyana_GWHPCBQA001310	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001310
actinidia_hemsleyana_GWHPCBQA001376	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001376
actinidia_hemsleyana_GWHPCBQA001393	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001393
actinidia_hemsleyana_GWHPCBQA001455	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001455
actinidia_hemsleyana_GWHPCBQA001557	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001557
actinidia_hemsleyana_GWHPCBQA001610	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001610
actinidia_hemsleyana_GWHPCBQA001611	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001611
actinidia_hemsleyana_GWHPCBQA001612	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001612
actinidia_hemsleyana_GWHPCBQA001651	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001651
actinidia_hemsleyana_GWHPCBQA001812	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001812
actinidia_hemsleyana_GWHPCBQA001846	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001846
actinidia_hemsleyana_GWHPCBQA001984	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA001984
actinidia_hemsleyana_GWHPCBQA002232	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA002232
actinidia_hemsleyana_GWHPCBQA002373	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA002373
actinidia_hemsleyana_GWHPCBQA002528	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA002528
actinidia_hemsleyana_GWHPCBQA002529	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA002529
actinidia_hemsleyana_GWHPCBQA002530	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA002530
actinidia_hemsleyana_GWHPCBQA002531	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA002531
actinidia_hemsleyana_GWHPCBQA002584	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA002584