Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_hemsleyana_GWHPCBQA002757	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA002757
actinidia_hemsleyana_GWHPCBQA003152	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA003152
actinidia_hemsleyana_GWHPCBQA003175	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA003175
actinidia_hemsleyana_GWHPCBQA003307	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA003307
actinidia_hemsleyana_GWHPCBQA003478	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA003478
actinidia_hemsleyana_GWHPCBQA003712	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA003712
actinidia_hemsleyana_GWHPCBQA003944	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA003944
actinidia_hemsleyana_GWHPCBQA003950	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA003950
actinidia_hemsleyana_GWHPCBQA003951	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA003951
actinidia_hemsleyana_GWHPCBQA003952	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA003952
actinidia_hemsleyana_GWHPCBQA004240	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA004240
actinidia_hemsleyana_GWHPCBQA004242	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA004242
actinidia_hemsleyana_GWHPCBQA004277	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA004277
actinidia_hemsleyana_GWHPCBQA004278	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA004278
actinidia_hemsleyana_GWHPCBQA004548	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA004548
actinidia_hemsleyana_GWHPCBQA004576	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA004576
actinidia_hemsleyana_GWHPCBQA004803	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA004803
actinidia_hemsleyana_GWHPCBQA004952	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA004952
actinidia_hemsleyana_GWHPCBQA004954	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA004954
actinidia_hemsleyana_GWHPCBQA005103	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA005103
actinidia_hemsleyana_GWHPCBQA005572	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA005572
actinidia_hemsleyana_GWHPCBQA005778	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA005778
actinidia_hemsleyana_GWHPCBQA005854	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA005854
actinidia_hemsleyana_GWHPCBQA005855	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA005855
actinidia_hemsleyana_GWHPCBQA005856	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA005856
actinidia_hemsleyana_GWHPCBQA005857	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA005857
actinidia_hemsleyana_GWHPCBQA005858	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA005858
actinidia_hemsleyana_GWHPCBQA005859	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA005859
actinidia_hemsleyana_GWHPCBQA005984	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA005984
actinidia_hemsleyana_GWHPCBQA005985	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA005985