Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_hemsleyana_GWHPCBQA005986	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA005986
actinidia_hemsleyana_GWHPCBQA005987	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA005987
actinidia_hemsleyana_GWHPCBQA006040	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA006040
actinidia_hemsleyana_GWHPCBQA006088	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA006088
actinidia_hemsleyana_GWHPCBQA006089	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA006089
actinidia_hemsleyana_GWHPCBQA006090	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA006090
actinidia_hemsleyana_GWHPCBQA006091	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA006091
actinidia_hemsleyana_GWHPCBQA006095	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA006095
actinidia_hemsleyana_GWHPCBQA006105	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA006105
actinidia_hemsleyana_GWHPCBQA006205	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA006205
actinidia_hemsleyana_GWHPCBQA006753	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA006753
actinidia_hemsleyana_GWHPCBQA006758	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA006758
actinidia_hemsleyana_GWHPCBQA006811	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA006811
actinidia_hemsleyana_GWHPCBQA006851	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA006851
actinidia_hemsleyana_GWHPCBQA006927	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA006927
actinidia_hemsleyana_GWHPCBQA006928	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA006928
actinidia_hemsleyana_GWHPCBQA006972	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA006972
actinidia_hemsleyana_GWHPCBQA007281	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA007281
actinidia_hemsleyana_GWHPCBQA007297	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA007297
actinidia_hemsleyana_GWHPCBQA007308	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA007308
actinidia_hemsleyana_GWHPCBQA007321	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA007321
actinidia_hemsleyana_GWHPCBQA007378	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA007378
actinidia_hemsleyana_GWHPCBQA007704	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA007704
actinidia_hemsleyana_GWHPCBQA007778	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA007778
actinidia_hemsleyana_GWHPCBQA007781	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA007781
actinidia_hemsleyana_GWHPCBQA007965	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA007965
actinidia_hemsleyana_GWHPCBQA008037	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA008037
actinidia_hemsleyana_GWHPCBQA008038	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA008038
actinidia_hemsleyana_GWHPCBQA008051	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA008051
actinidia_hemsleyana_GWHPCBQA008052	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA008052