Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_hemsleyana_GWHPCBQA008053	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA008053
actinidia_hemsleyana_GWHPCBQA008245	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA008245
actinidia_hemsleyana_GWHPCBQA008329	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA008329
actinidia_hemsleyana_GWHPCBQA008589	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA008589
actinidia_hemsleyana_GWHPCBQA008655	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA008655
actinidia_hemsleyana_GWHPCBQA009499	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA009499
actinidia_hemsleyana_GWHPCBQA009572	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA009572
actinidia_hemsleyana_GWHPCBQA009839	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA009839
actinidia_hemsleyana_GWHPCBQA010060	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA010060
actinidia_hemsleyana_GWHPCBQA010061	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA010061
actinidia_hemsleyana_GWHPCBQA010062	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA010062
actinidia_hemsleyana_GWHPCBQA010063	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA010063
actinidia_hemsleyana_GWHPCBQA010288	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA010288
actinidia_hemsleyana_GWHPCBQA010303	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA010303
actinidia_hemsleyana_GWHPCBQA010371	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA010371
actinidia_hemsleyana_GWHPCBQA011148	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA011148
actinidia_hemsleyana_GWHPCBQA011149	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA011149
actinidia_hemsleyana_GWHPCBQA011334	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA011334
actinidia_hemsleyana_GWHPCBQA011457	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA011457
actinidia_hemsleyana_GWHPCBQA011586	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA011586
actinidia_hemsleyana_GWHPCBQA011587	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA011587
actinidia_hemsleyana_GWHPCBQA011641	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA011641
actinidia_hemsleyana_GWHPCBQA011806	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA011806
actinidia_hemsleyana_GWHPCBQA011937	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA011937
actinidia_hemsleyana_GWHPCBQA011938	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA011938
actinidia_hemsleyana_GWHPCBQA012450	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA012450
actinidia_hemsleyana_GWHPCBQA012452	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA012452
actinidia_hemsleyana_GWHPCBQA012844	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA012844
actinidia_hemsleyana_GWHPCBQA013414	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA013414
actinidia_hemsleyana_GWHPCBQA013487	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA013487