Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_hemsleyana_GWHPCBQA013657	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA013657
actinidia_hemsleyana_GWHPCBQA013786	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA013786
actinidia_hemsleyana_GWHPCBQA013788	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA013788
actinidia_hemsleyana_GWHPCBQA013791	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA013791
actinidia_hemsleyana_GWHPCBQA013792	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA013792
actinidia_hemsleyana_GWHPCBQA013838	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA013838
actinidia_hemsleyana_GWHPCBQA014053	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA014053
actinidia_hemsleyana_GWHPCBQA014055	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA014055
actinidia_hemsleyana_GWHPCBQA014056	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA014056
actinidia_hemsleyana_GWHPCBQA014058	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA014058
actinidia_hemsleyana_GWHPCBQA014059	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA014059
actinidia_hemsleyana_GWHPCBQA014341	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA014341
actinidia_hemsleyana_GWHPCBQA014487	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA014487
actinidia_hemsleyana_GWHPCBQA014595	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA014595
actinidia_hemsleyana_GWHPCBQA014732	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA014732
actinidia_hemsleyana_GWHPCBQA014733	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA014733
actinidia_hemsleyana_GWHPCBQA014810	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA014810
actinidia_hemsleyana_GWHPCBQA014962	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA014962
actinidia_hemsleyana_GWHPCBQA014964	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA014964
actinidia_hemsleyana_GWHPCBQA014965	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA014965
actinidia_hemsleyana_GWHPCBQA014968	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA014968
actinidia_hemsleyana_GWHPCBQA015523	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA015523
actinidia_hemsleyana_GWHPCBQA015615	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA015615
actinidia_hemsleyana_GWHPCBQA015649	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA015649
actinidia_hemsleyana_GWHPCBQA015819	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA015819
actinidia_hemsleyana_GWHPCBQA016069	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016069
actinidia_hemsleyana_GWHPCBQA016145	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016145
actinidia_hemsleyana_GWHPCBQA016146	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016146
actinidia_hemsleyana_GWHPCBQA016149	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016149
actinidia_hemsleyana_GWHPCBQA016150	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016150