Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_hemsleyana_GWHPCBQA016214	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016214
actinidia_hemsleyana_GWHPCBQA016215	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016215
actinidia_hemsleyana_GWHPCBQA016429	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016429
actinidia_hemsleyana_GWHPCBQA016544	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016544
actinidia_hemsleyana_GWHPCBQA016560	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016560
actinidia_hemsleyana_GWHPCBQA016561	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016561
actinidia_hemsleyana_GWHPCBQA016567	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016567
actinidia_hemsleyana_GWHPCBQA016629	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016629
actinidia_hemsleyana_GWHPCBQA016630	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016630
actinidia_hemsleyana_GWHPCBQA016631	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016631
actinidia_hemsleyana_GWHPCBQA016637	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016637
actinidia_hemsleyana_GWHPCBQA016722	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016722
actinidia_hemsleyana_GWHPCBQA016724	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016724
actinidia_hemsleyana_GWHPCBQA016853	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016853
actinidia_hemsleyana_GWHPCBQA017354	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA017354
actinidia_hemsleyana_GWHPCBQA017523	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA017523
actinidia_hemsleyana_GWHPCBQA017980	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA017980
actinidia_hemsleyana_GWHPCBQA018119	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA018119
actinidia_hemsleyana_GWHPCBQA018410	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA018410
actinidia_hemsleyana_GWHPCBQA018612	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA018612
actinidia_hemsleyana_GWHPCBQA018656	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA018656
actinidia_hemsleyana_GWHPCBQA018764	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA018764
actinidia_hemsleyana_GWHPCBQA018765	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA018765
actinidia_hemsleyana_GWHPCBQA019038	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA019038
actinidia_hemsleyana_GWHPCBQA019168	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA019168
actinidia_hemsleyana_GWHPCBQA019397	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA019397
actinidia_hemsleyana_GWHPCBQA019398	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA019398
actinidia_hemsleyana_GWHPCBQA019464	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA019464
actinidia_hemsleyana_GWHPCBQA019617	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA019617
actinidia_hemsleyana_GWHPCBQA019676	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA019676