Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_hemsleyana_GWHPCBQA019782	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA019782
actinidia_hemsleyana_GWHPCBQA019996	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA019996
actinidia_hemsleyana_GWHPCBQA019997	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA019997
actinidia_hemsleyana_GWHPCBQA020001	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA020001
actinidia_hemsleyana_GWHPCBQA020197	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA020197
actinidia_hemsleyana_GWHPCBQA020209	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA020209
actinidia_hemsleyana_GWHPCBQA020379	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA020379
actinidia_hemsleyana_GWHPCBQA020710	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA020710
actinidia_hemsleyana_GWHPCBQA020850	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA020850
actinidia_hemsleyana_GWHPCBQA020851	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA020851
actinidia_hemsleyana_GWHPCBQA020852	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA020852
actinidia_hemsleyana_GWHPCBQA021113	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA021113
actinidia_hemsleyana_GWHPCBQA021490	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA021490
actinidia_hemsleyana_GWHPCBQA021712	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA021712
actinidia_hemsleyana_GWHPCBQA021713	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA021713
actinidia_hemsleyana_GWHPCBQA021971	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA021971
actinidia_hemsleyana_GWHPCBQA022059	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA022059
actinidia_hemsleyana_GWHPCBQA022786	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA022786
actinidia_hemsleyana_GWHPCBQA022903	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA022903
actinidia_hemsleyana_GWHPCBQA022906	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA022906
actinidia_hemsleyana_GWHPCBQA022913	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA022913
actinidia_hemsleyana_GWHPCBQA022932	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA022932
actinidia_hemsleyana_GWHPCBQA022933	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA022933
actinidia_hemsleyana_GWHPCBQA023222	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA023222
actinidia_hemsleyana_GWHPCBQA023224	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA023224
actinidia_hemsleyana_GWHPCBQA023263	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA023263
actinidia_hemsleyana_GWHPCBQA023266	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA023266
actinidia_hemsleyana_GWHPCBQA023294	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA023294
actinidia_hemsleyana_GWHPCBQA023297	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA023297
actinidia_hemsleyana_GWHPCBQA023450	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA023450