Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_hemsleyana_GWHPCBQA023535	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA023535
actinidia_hemsleyana_GWHPCBQA023536	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA023536
actinidia_hemsleyana_GWHPCBQA023875	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA023875
actinidia_hemsleyana_GWHPCBQA023976	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA023976
actinidia_hemsleyana_GWHPCBQA024009	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA024009
actinidia_hemsleyana_GWHPCBQA024312	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA024312
actinidia_hemsleyana_GWHPCBQA024506	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA024506
actinidia_hemsleyana_GWHPCBQA024559	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA024559
actinidia_hemsleyana_GWHPCBQA024560	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA024560
actinidia_hemsleyana_GWHPCBQA024643	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA024643
actinidia_hemsleyana_GWHPCBQA024789	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA024789
actinidia_hemsleyana_GWHPCBQA024823	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA024823
actinidia_hemsleyana_GWHPCBQA024891	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA024891
actinidia_hemsleyana_GWHPCBQA024928	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA024928
actinidia_hemsleyana_GWHPCBQA024989	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA024989
actinidia_hemsleyana_GWHPCBQA024990	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA024990
actinidia_hemsleyana_GWHPCBQA024991	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA024991
actinidia_hemsleyana_GWHPCBQA024992	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA024992
actinidia_hemsleyana_GWHPCBQA025136	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA025136
actinidia_hemsleyana_GWHPCBQA025137	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA025137
actinidia_hemsleyana_GWHPCBQA025255	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA025255
actinidia_hemsleyana_GWHPCBQA025280	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA025280
actinidia_hemsleyana_GWHPCBQA025378	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA025378
actinidia_hemsleyana_GWHPCBQA025549	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA025549
actinidia_hemsleyana_GWHPCBQA025625	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA025625
actinidia_hemsleyana_GWHPCBQA025674	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA025674
actinidia_hemsleyana_GWHPCBQA025972	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA025972
actinidia_hemsleyana_GWHPCBQA026132	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA026132
actinidia_hemsleyana_GWHPCBQA026144	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA026144
actinidia_hemsleyana_GWHPCBQA026222	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA026222