Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_hemsleyana_GWHPCBQA026260	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA026260
actinidia_hemsleyana_GWHPCBQA026309	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA026309
actinidia_hemsleyana_GWHPCBQA026334	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA026334
actinidia_hemsleyana_GWHPCBQA026355	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA026355
actinidia_hemsleyana_GWHPCBQA026523	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA026523
actinidia_hemsleyana_GWHPCBQA026524	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA026524
actinidia_hemsleyana_GWHPCBQA026525	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA026525
actinidia_hemsleyana_GWHPCBQA026638	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA026638
actinidia_hemsleyana_GWHPCBQA026639	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA026639
actinidia_hemsleyana_GWHPCBQA027129	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA027129
actinidia_hemsleyana_GWHPCBQA027163	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA027163
actinidia_hemsleyana_GWHPCBQA027165	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA027165
actinidia_hemsleyana_GWHPCBQA027214	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA027214
actinidia_hemsleyana_GWHPCBQA027251	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA027251
actinidia_hemsleyana_GWHPCBQA027451	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA027451
actinidia_hemsleyana_GWHPCBQA027498	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA027498
actinidia_hemsleyana_GWHPCBQA027499	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA027499
actinidia_hemsleyana_GWHPCBQA027500	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA027500
actinidia_hemsleyana_GWHPCBQA027507	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA027507
actinidia_hemsleyana_GWHPCBQA028186	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028186
actinidia_hemsleyana_GWHPCBQA028187	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028187
actinidia_hemsleyana_GWHPCBQA028193	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028193
actinidia_hemsleyana_GWHPCBQA028194	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028194
actinidia_hemsleyana_GWHPCBQA028195	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028195
actinidia_hemsleyana_GWHPCBQA028196	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028196
actinidia_hemsleyana_GWHPCBQA028222	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028222
actinidia_hemsleyana_GWHPCBQA028223	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028223
actinidia_hemsleyana_GWHPCBQA028224	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028224
actinidia_hemsleyana_GWHPCBQA028264	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028264
actinidia_hemsleyana_GWHPCBQA028270	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028270