Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_hemsleyana_GWHPCBQA028325	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028325
actinidia_hemsleyana_GWHPCBQA028584	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028584
actinidia_hemsleyana_GWHPCBQA028763	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028763
actinidia_hemsleyana_GWHPCBQA028792	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028792
actinidia_hemsleyana_GWHPCBQA028820	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028820
actinidia_hemsleyana_GWHPCBQA028821	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028821
actinidia_hemsleyana_GWHPCBQA028845	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028845
actinidia_hemsleyana_GWHPCBQA028855	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA028855
actinidia_hemsleyana_GWHPCBQA029459	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA029459
actinidia_hemsleyana_GWHPCBQA029592	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA029592
actinidia_hemsleyana_GWHPCBQA029723	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA029723
actinidia_hemsleyana_GWHPCBQA029813	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA029813
actinidia_hemsleyana_GWHPCBQA030105	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA030105
actinidia_hemsleyana_GWHPCBQA030238	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA030238
actinidia_hemsleyana_GWHPCBQA030239	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA030239
actinidia_hemsleyana_GWHPCBQA030240	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA030240
actinidia_hemsleyana_GWHPCBQA030293	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA030293
actinidia_hemsleyana_GWHPCBQA030362	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA030362
actinidia_hemsleyana_GWHPCBQA030363	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA030363
actinidia_hemsleyana_GWHPCBQA030482	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA030482
actinidia_hemsleyana_GWHPCBQA030699	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA030699
actinidia_hemsleyana_GWHPCBQA031219	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA031219
actinidia_hemsleyana_GWHPCBQA031220	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA031220
actinidia_hemsleyana_GWHPCBQA031221	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA031221
actinidia_hemsleyana_GWHPCBQA031222	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA031222
actinidia_hemsleyana_GWHPCBQA031223	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA031223
actinidia_hemsleyana_GWHPCBQA031224	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA031224
actinidia_hemsleyana_GWHPCBQA031225	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA031225
actinidia_hemsleyana_GWHPCBQA031227	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA031227
actinidia_hemsleyana_GWHPCBQA031229	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA031229