Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_hemsleyana_GWHPCBQA031231	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA031231
actinidia_hemsleyana_GWHPCBQA031240	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA031240
actinidia_hemsleyana_GWHPCBQA031368	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA031368
actinidia_hemsleyana_GWHPCBQA031601	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA031601
actinidia_hemsleyana_GWHPCBQA031662	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA031662
actinidia_hemsleyana_GWHPCBQA032383	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA032383
actinidia_hemsleyana_GWHPCBQA032550	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA032550
actinidia_hemsleyana_GWHPCBQA032647	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA032647
actinidia_hemsleyana_GWHPCBQA032648	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA032648
actinidia_hemsleyana_GWHPCBQA032649	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA032649
actinidia_hemsleyana_GWHPCBQA032699	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA032699
actinidia_hemsleyana_GWHPCBQA032706	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA032706
actinidia_hemsleyana_GWHPCBQA032785	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA032785
actinidia_hemsleyana_GWHPCBQA032786	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA032786
actinidia_hemsleyana_GWHPCBQA032787	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA032787
actinidia_hemsleyana_GWHPCBQA032857	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA032857
actinidia_hemsleyana_GWHPCBQA032916	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA032916
actinidia_hemsleyana_GWHPCBQA033603	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA033603
actinidia_hemsleyana_GWHPCBQA033661	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA033661
actinidia_hemsleyana_GWHPCBQA033734	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA033734
actinidia_hemsleyana_GWHPCBQA033735	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA033735
actinidia_hemsleyana_GWHPCBQA033781	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA033781
actinidia_hemsleyana_GWHPCBQA033843	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA033843
actinidia_hemsleyana_GWHPCBQA033889	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA033889
actinidia_hemsleyana_GWHPCBQA034166	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA034166
actinidia_hemsleyana_GWHPCBQA034199	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA034199
actinidia_hemsleyana_GWHPCBQA034262	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA034262
actinidia_hemsleyana_GWHPCBQA034263	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA034263
actinidia_hemsleyana_GWHPCBQA034558	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA034558
actinidia_hemsleyana_GWHPCBQA034772	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA034772