Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_hemsleyana_GWHPCBQA035264	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA035264
actinidia_hemsleyana_GWHPCBQA035265	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA035265
actinidia_hemsleyana_GWHPCBQA035266	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA035266
actinidia_hemsleyana_GWHPCBQA035302	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA035302
actinidia_hemsleyana_GWHPCBQA035303	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA035303
actinidia_hemsleyana_GWHPCBQA035761	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA035761
actinidia_hemsleyana_GWHPCBQA035762	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA035762
actinidia_hemsleyana_GWHPCBQA035763	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA035763
actinidia_hemsleyana_GWHPCBQA035764	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA035764
actinidia_hemsleyana_GWHPCBQA035765	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA035765
actinidia_hemsleyana_GWHPCBQA035766	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA035766
actinidia_hemsleyana_GWHPCBQA035767	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA035767
actinidia_hemsleyana_GWHPCBQA035832	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA035832
actinidia_hemsleyana_GWHPCBQA035845	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA035845
actinidia_hemsleyana_GWHPCBQA036111	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA036111
actinidia_hemsleyana_GWHPCBQA036257	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA036257
actinidia_hemsleyana_GWHPCBQA036304	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA036304
actinidia_hemsleyana_GWHPCBQA036589	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA036589
actinidia_hemsleyana_GWHPCBQA036591	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA036591
actinidia_hemsleyana_GWHPCBQA036592	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA036592
actinidia_hemsleyana_GWHPCBQA036593	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA036593
actinidia_hemsleyana_GWHPCBQA036594	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA036594
actinidia_hemsleyana_GWHPCBQA036652	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA036652
actinidia_hemsleyana_GWHPCBQA036771	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA036771
actinidia_hemsleyana_GWHPCBQA037025	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA037025
actinidia_hemsleyana_GWHPCBQA037217	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA037217
actinidia_hemsleyana_GWHPCBQA037218	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA037218
actinidia_hemsleyana_GWHPCBQA037221	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA037221
actinidia_hemsleyana_GWHPCBQA037222	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA037222
actinidia_hemsleyana_GWHPCBQA037223	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA037223