Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_hemsleyana_GWHPCBQA037438	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA037438
actinidia_hemsleyana_GWHPCBQA037465	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA037465
actinidia_hemsleyana_GWHPCBQA037542	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA037542
actinidia_hemsleyana_GWHPCBQA037824	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA037824
actinidia_hemsleyana_GWHPCBQA037942	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA037942
actinidia_hemsleyana_GWHPCBQA038215	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA038215
actinidia_hemsleyana_GWHPCBQA038375	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA038375
actinidia_hemsleyana_GWHPCBQA038664	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA038664
actinidia_hemsleyana_GWHPCBQA038685	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA038685
actinidia_hemsleyana_GWHPCBQA038773	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA038773
actinidia_hemsleyana_GWHPCBQA038944	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA038944
actinidia_hemsleyana_GWHPCBQA038945	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA038945
actinidia_hemsleyana_GWHPCBQA038983	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA038983
actinidia_hemsleyana_GWHPCBQA039140	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA039140
actinidia_hemsleyana_GWHPCBQA039298	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA039298
actinidia_hemsleyana_GWHPCBQA039311	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA039311
actinidia_hemsleyana_GWHPCBQA039626	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA039626
actinidia_hemsleyana_GWHPCBQA039806	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA039806
actinidia_hemsleyana_GWHPCBQA039813	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA039813
actinidia_hemsleyana_GWHPCBQA039814	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA039814
actinidia_hemsleyana_GWHPCBQA039824	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA039824
actinidia_hemsleyana_GWHPCBQA039944	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA039944
actinidia_hemsleyana_GWHPCBQA039945	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA039945
actinidia_hemsleyana_GWHPCBQA039995	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA039995
actinidia_hemsleyana_GWHPCBQA040030	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040030
actinidia_hemsleyana_GWHPCBQA040033	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040033
actinidia_hemsleyana_GWHPCBQA040057	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040057
actinidia_hemsleyana_GWHPCBQA040058	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040058
actinidia_hemsleyana_GWHPCBQA040085	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040085
actinidia_hemsleyana_GWHPCBQA040087	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040087