Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_hemsleyana_GWHPCBQA040090	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040090
actinidia_hemsleyana_GWHPCBQA040205	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040205
actinidia_hemsleyana_GWHPCBQA040276	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040276
actinidia_hemsleyana_GWHPCBQA040330	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040330
actinidia_hemsleyana_GWHPCBQA040331	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040331
actinidia_hemsleyana_GWHPCBQA040332	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040332
actinidia_hemsleyana_GWHPCBQA040333	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040333
actinidia_hemsleyana_GWHPCBQA040514	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040514
actinidia_hemsleyana_GWHPCBQA040677	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040677
actinidia_hemsleyana_GWHPCBQA040756	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040756
actinidia_hemsleyana_GWHPCBQA040947	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040947
actinidia_hemsleyana_GWHPCBQA040957	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA040957
actinidia_hemsleyana_GWHPCBQA041060	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA041060
actinidia_hemsleyana_GWHPCBQA041132	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA041132
actinidia_hemsleyana_GWHPCBQA041183	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA041183
actinidia_hemsleyana_GWHPCBQA041227	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA041227
actinidia_hemsleyana_GWHPCBQA041743	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA041743
actinidia_hemsleyana_GWHPCBQA042296	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA042296
actinidia_hemsleyana_GWHPCBQA042646	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA042646
actinidia_hemsleyana_GWHPCBQA042717	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA042717
actinidia_hemsleyana_GWHPCBQA042896	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA042896
actinidia_hemsleyana_GWHPCBQA042897	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA042897
actinidia_hemsleyana_GWHPCBQA042965	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA042965
actinidia_hemsleyana_GWHPCBQA043375	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA043375
actinidia_hemsleyana_GWHPCBQA043384	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA043384
actinidia_hemsleyana_GWHPCBQA043387	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA043387
actinidia_hemsleyana_GWHPCBQA043637	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA043637
actinidia_hemsleyana_GWHPCBQA043638	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA043638
actinidia_hemsleyana_GWHPCBQA043639	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA043639
actinidia_hemsleyana_GWHPCBQA043640	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA043640