Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_hemsleyana_GWHPCBQA044117	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA044117
actinidia_hemsleyana_GWHPCBQA044209	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA044209
actinidia_hemsleyana_GWHPCBQA044212	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA044212
actinidia_hemsleyana_GWHPCBQA044213	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA044213
actinidia_hemsleyana_GWHPCBQA044214	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA044214
actinidia_hemsleyana_GWHPCBQA044215	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA044215
actinidia_hemsleyana_GWHPCBQA044217	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA044217
actinidia_hemsleyana_GWHPCBQA044218	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA044218
actinidia_hemsleyana_GWHPCBQA044221	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA044221
actinidia_hemsleyana_GWHPCBQA044223	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA044223
actinidia_hemsleyana_GWHPCBQA044239	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA044239
actinidia_hemsleyana_GWHPCBQA044240	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA044240
actinidia_hemsleyana_GWHPCBQA044243	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA044243
actinidia_hemsleyana_GWHPCBQA044361	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA044361
actinidia_hemsleyana_GWHPCBQA044685	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA044685
actinidia_hemsleyana_GWHPCBQA044920	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA044920
actinidia_hemsleyana_GWHPCBQA045358	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA045358
actinidia_hemsleyana_GWHPCBQA045431	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA045431
actinidia_hemsleyana_GWHPCBQA045459	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA045459
actinidia_hemsleyana_GWHPCBQA045478	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA045478
actinidia_hemsleyana_GWHPCBQA045813	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA045813
actinidia_hemsleyana_GWHPCBQA045889	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA045889
actinidia_hemsleyana_GWHPCBQA045912	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA045912
actinidia_hemsleyana_GWHPCBQA045915	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA045915
actinidia_hemsleyana_GWHPCBQA045916	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA045916
actinidia_hemsleyana_GWHPCBQA045966	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA045966
actinidia_hemsleyana_GWHPCBQA046063	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA046063
actinidia_hemsleyana_GWHPCBQA046324	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA046324
actinidia_hemsleyana_GWHPCBQA046378	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA046378
actinidia_hemsleyana_GWHPCBQA046379	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA046379