Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_zhejiangensis_GWHPCBQB000184	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB000184
actinidia_zhejiangensis_GWHPCBQB000549	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB000549
actinidia_zhejiangensis_GWHPCBQB001085	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001085
actinidia_zhejiangensis_GWHPCBQB001175	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001175
actinidia_zhejiangensis_GWHPCBQB001222	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001222
actinidia_zhejiangensis_GWHPCBQB001229	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001229
actinidia_zhejiangensis_GWHPCBQB001237	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001237
actinidia_zhejiangensis_GWHPCBQB001365	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001365
actinidia_zhejiangensis_GWHPCBQB001366	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001366
actinidia_zhejiangensis_GWHPCBQB001422	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001422
actinidia_zhejiangensis_GWHPCBQB001505	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001505
actinidia_zhejiangensis_GWHPCBQB001506	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001506
actinidia_zhejiangensis_GWHPCBQB001526	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001526
actinidia_zhejiangensis_GWHPCBQB001593	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001593
actinidia_zhejiangensis_GWHPCBQB001695	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001695
actinidia_zhejiangensis_GWHPCBQB001753	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001753
actinidia_zhejiangensis_GWHPCBQB001754	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001754
actinidia_zhejiangensis_GWHPCBQB001783	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001783
actinidia_zhejiangensis_GWHPCBQB001942	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001942
actinidia_zhejiangensis_GWHPCBQB001974	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB001974
actinidia_zhejiangensis_GWHPCBQB002128	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB002128
actinidia_zhejiangensis_GWHPCBQB002538	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB002538
actinidia_zhejiangensis_GWHPCBQB002623	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB002623
actinidia_zhejiangensis_GWHPCBQB002707	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB002707
actinidia_zhejiangensis_GWHPCBQB002708	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB002708
actinidia_zhejiangensis_GWHPCBQB002709	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB002709
actinidia_zhejiangensis_GWHPCBQB002756	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB002756
actinidia_zhejiangensis_GWHPCBQB002960	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB002960
actinidia_zhejiangensis_GWHPCBQB003380	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB003380
actinidia_zhejiangensis_GWHPCBQB003522	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB003522