Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_zhejiangensis_GWHPCBQB003695	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB003695
actinidia_zhejiangensis_GWHPCBQB003919	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB003919
actinidia_zhejiangensis_GWHPCBQB004156	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB004156
actinidia_zhejiangensis_GWHPCBQB004161	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB004161
actinidia_zhejiangensis_GWHPCBQB004430	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB004430
actinidia_zhejiangensis_GWHPCBQB004433	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB004433
actinidia_zhejiangensis_GWHPCBQB004469	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB004469
actinidia_zhejiangensis_GWHPCBQB004550	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB004550
actinidia_zhejiangensis_GWHPCBQB004772	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB004772
actinidia_zhejiangensis_GWHPCBQB004795	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB004795
actinidia_zhejiangensis_GWHPCBQB005025	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB005025
actinidia_zhejiangensis_GWHPCBQB005208	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB005208
actinidia_zhejiangensis_GWHPCBQB005209	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB005209
actinidia_zhejiangensis_GWHPCBQB005353	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB005353
actinidia_zhejiangensis_GWHPCBQB005417	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB005417
actinidia_zhejiangensis_GWHPCBQB005819	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB005819
actinidia_zhejiangensis_GWHPCBQB005952	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB005952
actinidia_zhejiangensis_GWHPCBQB006053	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB006053
actinidia_zhejiangensis_GWHPCBQB006059	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB006059
actinidia_zhejiangensis_GWHPCBQB006155	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB006155
actinidia_zhejiangensis_GWHPCBQB006156	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB006156
actinidia_zhejiangensis_GWHPCBQB006157	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB006157
actinidia_zhejiangensis_GWHPCBQB006158	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB006158
actinidia_zhejiangensis_GWHPCBQB006159	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB006159
actinidia_zhejiangensis_GWHPCBQB006160	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB006160
actinidia_zhejiangensis_GWHPCBQB006161	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB006161
actinidia_zhejiangensis_GWHPCBQB006286	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB006286
actinidia_zhejiangensis_GWHPCBQB006287	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB006287
actinidia_zhejiangensis_GWHPCBQB006288	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB006288
actinidia_zhejiangensis_GWHPCBQB006289	actinidia_zhejiangensis	actinidia_zhejiangensis_GWHPCBQB006289