PlantSME Plant secondary metabolism enzyme database
Rosmarinic acid portable pathway

Rosmarinic acid portable pathway

Category：Rosmarinic acid pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route
Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.
7 curated steps/modules
Phenylalanine Donor Branch
01
Substrate
L-Phenylalanine
C9H11NO2
C6H5-CH2-CH(NH2)-COOH
Deamination into the phenylpropanoid donor branch
PAL7015
Product
trans-Cinnamic acid
C9H8O2
C6H5-CH=CH-COOH
PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.
02
Substrate
trans-Cinnamic acid
C9H8O2
C6H5-CH=CH-COOH
Cinnamate 4-hydroxylation
C4H48992
Product
4-Coumaric acid
C9H8O3
4-HO-C6H4-CH=CH-COOH
C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.
03
Substrate
4-Coumaric acid
C9H8O3
4-HO-C6H4-CH=CH-COOH
Substrate
CoA
C21H36N7O16P3S
CoA-SH
CoA ligation to form the RAS acyl donor
4CL25914
Product
4-Coumaroyl-CoA
C30H38N7O18P3S
4-HO-C6H4-CH=CH-C(=O)-SCoA
4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.
Tyrosine Acceptor Branch And Condensation
04
Substrate
L-Tyrosine
C9H11NO3
4-HO-C6H4-CH2-CH(NH2)-COOH
Transamination to 4-hydroxyphenylpyruvate
TAT14219
Product
4-Hydroxyphenylpyruvate
C9H8O4
4-HO-C6H4-CH2-C(=O)-COOH
TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.
05
Substrate
4-Hydroxyphenylpyruvate
C9H8O4
4-HO-C6H4-CH2-C(=O)-COOH
Reduction to the HPLA acceptor
HPPR5065
Product
4-Hydroxyphenyllactate
C9H10O4
4-HO-C6H4-CH2-CH(OH)-COOH
HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.
06
Substrate
4-Coumaroyl-CoA
C30H38N7O18P3S
4-HO-C6H4-CH=CH-C(=O)-SCoA
Substrate
4-Hydroxyphenyllactate
C9H10O4
4-HO-C6H4-CH2-CH(OH)-COOH
Ester-forming condensation
RAS13433
Product
4-Coumaroyl-4'-hydroxyphenyllactate
C18H16O7
4-HO-C6H4-CH=CH-C(=O)-O-CH(COOH)-CH2-Ar-OH
RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.
07
Substrate
4-Coumaroyl-4'-hydroxyphenyllactate
C18H16O7
mono-catechol ester precursor
CYP98A14-type hydroxylation with CPR electron-transfer support
CPR5870
Product
Rosmarinic acid
C18H16O8
caffeoyl-O-3,4-dihydroxyphenyllactate
CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.
Lineage Distribution
Assignments under this module are distributed across the following plant lineages.
All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)
Algae
Hits388
Bryophytes
Hits9418
Lycophytes/Ferns
Hits423
Gymnosperms
Hits1285
Basal angiosperms
Hits2118
Monocots
Hits23710
Eudicots
Hits83094
Species Distribution
Triticum turgidum
Aegilops tauschii
Hordeum vulgare
Triticum aestivum
Prunus domestica
Ocimum basilicum
Avena sativa
Papaver setigerum
Pogostemon cablin
Fragaria ananassa
Lineage Summary
Algae
388
Bryophytes
9418
Lycophytes/Ferns
423
Gymnosperms
1285
Basal angiosperms
2118
Monocots
23710
Eudicots
83094
Module Gene List
GeneSpeciesProtein ID
XP_027368795.1 abrus_precatorius XP_027368795.1
XP_027368814.1 abrus_precatorius XP_027368814.1
XP_027368868.1 abrus_precatorius XP_027368868.1
Aalbaalba5_s000005250004900.1 abies_alba Aalbaalba5_s000005250004900.1
Aalbaalba5_s000005250004900.2 abies_alba Aalbaalba5_s000005250004900.2
Aalbaalba5_s000010050009620.1 abies_alba Aalbaalba5_s000010050009620.1
Aalbaalba5_s000011330010450.1 abies_alba Aalbaalba5_s000011330010450.1
Aalbaalba5_s000012040011080.1 abies_alba Aalbaalba5_s000012040011080.1
Aalbaalba5_s000025860022090.1 abies_alba Aalbaalba5_s000025860022090.1
Aalbaalba5_s000029260024580.1 abies_alba Aalbaalba5_s000029260024580.1
Aalbaalba5_s000032090026800.1 abies_alba Aalbaalba5_s000032090026800.1
Aalbaalba5_s000058480044550.1 abies_alba Aalbaalba5_s000058480044550.1
Aalbaalba5_s000096760068470.1 abies_alba Aalbaalba5_s000096760068470.1
Aalbaalba5_s000103830072450.1 abies_alba Aalbaalba5_s000103830072450.1
Aalbaalba5_s000104320072590.1 abies_alba Aalbaalba5_s000104320072590.1
Aalbaalba5_s000110210075580.1 abies_alba Aalbaalba5_s000110210075580.1
Aalbaalba5_s000116070078590.1 abies_alba Aalbaalba5_s000116070078590.1
Aalbaalba5_s000137720089890.1 abies_alba Aalbaalba5_s000137720089890.1
Aalbaalba5_s000142160092290.1 abies_alba Aalbaalba5_s000142160092290.1
Aalbaalba5_s000166900105230.2 abies_alba Aalbaalba5_s000166900105230.2
Aalbaalba5_s000180540112240.1 abies_alba Aalbaalba5_s000180540112240.1
Aalbaalba5_s000180920112470.1 abies_alba Aalbaalba5_s000180920112470.1
Aalbaalba5_s000180920112480.1 abies_alba Aalbaalba5_s000180920112480.1
Aalbaalba5_s000200280122070.1 abies_alba Aalbaalba5_s000200280122070.1
Aalbaalba5_s000218160130150.1 abies_alba Aalbaalba5_s000218160130150.1
Aalbaalba5_s000222700132120.1 abies_alba Aalbaalba5_s000222700132120.1
Aalbaalba5_s000230930135980.1 abies_alba Aalbaalba5_s000230930135980.1
Aalbaalba5_s000261240149780.1 abies_alba Aalbaalba5_s000261240149780.1
Aalbaalba5_s000298500165700.1 abies_alba Aalbaalba5_s000298500165700.1
Aalbaalba5_s000306150168840.1 abies_alba Aalbaalba5_s000306150168840.1