Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT14389 SQE4569 CAS35857

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT327781 SMO1/SMO24067 CPI11261 CYP51G1190 FK1234 HYD11364 DWF71524 DWF5888 DWF11689

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET21316 DWF4/CYP90B1290 CPD/CYP90A1103 ROT3/CYP90C1193 CYP90D1142 BR6OX1/CYP85A140 BR6OX2/CYP85A268

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (569) Bryophytes (5698) Lycophytes/Ferns (189) Gymnosperms (791) Basal angiosperms (1411) Monocots (17019) Eudicots (61245)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
alstonia_scholaris_tjs31736	alstonia_scholaris	alstonia_scholaris_tjs31736
alstonia_scholaris_tjs31744	alstonia_scholaris	alstonia_scholaris_tjs31744
alstonia_scholaris_tjs32909	alstonia_scholaris	alstonia_scholaris_tjs32909
alstonia_scholaris_tjs32917	alstonia_scholaris	alstonia_scholaris_tjs32917
alstonia_scholaris_tjs33009	alstonia_scholaris	alstonia_scholaris_tjs33009
alstonia_scholaris_tjs34733	alstonia_scholaris	alstonia_scholaris_tjs34733
alstonia_scholaris_tjs35173	alstonia_scholaris	alstonia_scholaris_tjs35173
alstonia_scholaris_tjs35586	alstonia_scholaris	alstonia_scholaris_tjs35586
alstonia_scholaris_tjs35710	alstonia_scholaris	alstonia_scholaris_tjs35710
alstonia_scholaris_tjs35969	alstonia_scholaris	alstonia_scholaris_tjs35969
alstonia_scholaris_tjs35976	alstonia_scholaris	alstonia_scholaris_tjs35976
alstonia_scholaris_tjs37075	alstonia_scholaris	alstonia_scholaris_tjs37075
alstonia_scholaris_tjs37511	alstonia_scholaris	alstonia_scholaris_tjs37511
alstonia_scholaris_tjs37581	alstonia_scholaris	alstonia_scholaris_tjs37581
alstonia_scholaris_tjs37606	alstonia_scholaris	alstonia_scholaris_tjs37606
AcruScioBoG_102831_HRSCAF_1030440003830.1	amaranthus_cruentus	AcruScioBoG_102831_HRSCAF_1030440003830.1
AcruScioBoG_102831_HRSCAF_1030440007210.1	amaranthus_cruentus	AcruScioBoG_102831_HRSCAF_1030440007210.1
AcruScioBoG_102833_HRSCAF_1030520013560.1	amaranthus_cruentus	AcruScioBoG_102833_HRSCAF_1030520013560.1
AcruScioBoG_102835_HRSCAF_1030590021460.1	amaranthus_cruentus	AcruScioBoG_102835_HRSCAF_1030590021460.1
AcruScioBoG_102835_HRSCAF_1030590025280.1	amaranthus_cruentus	AcruScioBoG_102835_HRSCAF_1030590025280.1
AcruScioBoG_102836_HRSCAF_1030720030580.1	amaranthus_cruentus	AcruScioBoG_102836_HRSCAF_1030720030580.1
AcruScioBoG_102837_HRSCAF_1030740031950.1	amaranthus_cruentus	AcruScioBoG_102837_HRSCAF_1030740031950.1
AcruScioBoG_102837_HRSCAF_1030740031950.3	amaranthus_cruentus	AcruScioBoG_102837_HRSCAF_1030740031950.3
AcruScioBoG_102837_HRSCAF_1030740033520.1	amaranthus_cruentus	AcruScioBoG_102837_HRSCAF_1030740033520.1
AcruScioBoG_102838_HRSCAF_1030810040050.1	amaranthus_cruentus	AcruScioBoG_102838_HRSCAF_1030810040050.1
AcruScioBoG_102838_HRSCAF_1030810040820.1	amaranthus_cruentus	AcruScioBoG_102838_HRSCAF_1030810040820.1
AcruScioBoG_102838_HRSCAF_1030810040820.2	amaranthus_cruentus	AcruScioBoG_102838_HRSCAF_1030810040820.2
AcruScioBoG_102838_HRSCAF_1030810041960.1	amaranthus_cruentus	AcruScioBoG_102838_HRSCAF_1030810041960.1
AcruScioBoG_102838_HRSCAF_1030810044070.1	amaranthus_cruentus	AcruScioBoG_102838_HRSCAF_1030810044070.1
AcruScioBoG_102838_HRSCAF_1030810044070.2	amaranthus_cruentus	AcruScioBoG_102838_HRSCAF_1030810044070.2