Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT14389 SQE4569 CAS35857

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT327781 SMO1/SMO24067 CPI11261 CYP51G1190 FK1234 HYD11364 DWF71524 DWF5888 DWF11689

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET21316 DWF4/CYP90B1290 CPD/CYP90A1103 ROT3/CYP90C1193 CYP90D1142 BR6OX1/CYP85A140 BR6OX2/CYP85A268

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (569) Bryophytes (5698) Lycophytes/Ferns (189) Gymnosperms (791) Basal angiosperms (1411) Monocots (17019) Eudicots (61245)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
Amac1672290101810	andreaeobryum_macrosporum	Amac1672290101810
Amac1673830103910	andreaeobryum_macrosporum	Amac1673830103910
Amac1674860105270	andreaeobryum_macrosporum	Amac1674860105270
Amac1674890105360	andreaeobryum_macrosporum	Amac1674890105360
Amac1678400110930	andreaeobryum_macrosporum	Amac1678400110930
Amac1682010117750	andreaeobryum_macrosporum	Amac1682010117750
Amac1683250120340	andreaeobryum_macrosporum	Amac1683250120340
Amac1684190122310	andreaeobryum_macrosporum	Amac1684190122310
Amac843870135070	andreaeobryum_macrosporum	Amac843870135070
Amac879300136750	andreaeobryum_macrosporum	Amac879300136750
Amac910230137940	andreaeobryum_macrosporum	Amac910230137940
Amac919200138550	andreaeobryum_macrosporum	Amac919200138550
Amac976760144860	andreaeobryum_macrosporum	Amac976760144860
andrographis_paniculata_XP_051113301.1	andrographis_paniculata	andrographis_paniculata_XP_051113301.1
andrographis_paniculata_XP_051113919.1	andrographis_paniculata	andrographis_paniculata_XP_051113919.1
andrographis_paniculata_XP_051113920.1	andrographis_paniculata	andrographis_paniculata_XP_051113920.1
andrographis_paniculata_XP_051113921.1	andrographis_paniculata	andrographis_paniculata_XP_051113921.1
andrographis_paniculata_XP_051113922.1	andrographis_paniculata	andrographis_paniculata_XP_051113922.1
andrographis_paniculata_XP_051113924.1	andrographis_paniculata	andrographis_paniculata_XP_051113924.1
andrographis_paniculata_XP_051113925.1	andrographis_paniculata	andrographis_paniculata_XP_051113925.1
andrographis_paniculata_XP_051113926.1	andrographis_paniculata	andrographis_paniculata_XP_051113926.1
andrographis_paniculata_XP_051113927.1	andrographis_paniculata	andrographis_paniculata_XP_051113927.1
andrographis_paniculata_XP_051113928.1	andrographis_paniculata	andrographis_paniculata_XP_051113928.1
andrographis_paniculata_XP_051113929.1	andrographis_paniculata	andrographis_paniculata_XP_051113929.1
andrographis_paniculata_XP_051113930.1	andrographis_paniculata	andrographis_paniculata_XP_051113930.1
andrographis_paniculata_XP_051113931.1	andrographis_paniculata	andrographis_paniculata_XP_051113931.1
andrographis_paniculata_XP_051113932.1	andrographis_paniculata	andrographis_paniculata_XP_051113932.1
andrographis_paniculata_XP_051113933.1	andrographis_paniculata	andrographis_paniculata_XP_051113933.1
andrographis_paniculata_XP_051113934.1	andrographis_paniculata	andrographis_paniculata_XP_051113934.1
andrographis_paniculata_XP_051114812.1	andrographis_paniculata	andrographis_paniculata_XP_051114812.1