Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT190 SQE37 CAS726

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT3593 SMO1/SMO2208 CPI118 CYP51G1179 FK18 HYD122 DWF7106 DWF55 DWF128

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET250 DWF4/CYP90B157 CPD/CYP90A163 ROT3/CYP90C110 CYP90D11 BR6OX1/CYP85A19 BR6OX2/CYP85A24

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Basal angiosperms (2224)

Current lineage filter: Basal angiosperms. All species, family, module, and gene statistics below have been narrowed to this lineage.

Species Distribution

Chloranthus sessilifolius

Amborella trichopoda

Piper nigrum

Aristolochia contorta

Nymphaea colorata

Liriodendron chinense

Lineage Summary

Basal angiosperms

2224

Module Gene List

Gene	Species	Protein ID
AtriAmTr_v1.0_scaffold000840190430.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000840190430.1
AtriAmTr_v1.0_scaffold000890196020.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000890196020.1
AtriAmTr_v1.0_scaffold000890196030.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000890196030.1
AtriAmTr_v1.0_scaffold000890196040.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000890196040.1
AtriAmTr_v1.0_scaffold000890196050.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000890196050.1
AtriAmTr_v1.0_scaffold000890196080.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000890196080.1
AtriAmTr_v1.0_scaffold000890196200.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000890196200.1
AtriAmTr_v1.0_scaffold000890196210.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000890196210.1
AtriAmTr_v1.0_scaffold001030208900.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001030208900.1
AtriAmTr_v1.0_scaffold001030209200.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001030209200.1
AtriAmTr_v1.0_scaffold001030209210.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001030209210.1
AtriAmTr_v1.0_scaffold001030209220.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001030209220.1
AtriAmTr_v1.0_scaffold001170220550.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001170220550.1
AtriAmTr_v1.0_scaffold001190221680.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001190221680.1
AtriAmTr_v1.0_scaffold001330229780.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001330229780.1
AtriAmTr_v1.0_scaffold001400233440.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001400233440.1
AtriAmTr_v1.0_scaffold001400233450.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001400233450.1
AtriAmTr_v1.0_scaffold001530240220.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001530240220.1
AtriAmTr_v1.0_scaffold001540240630.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001540240630.1
AtriAmTr_v1.0_scaffold001560242230.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001560242230.1
AtriAmTr_v1.0_scaffold001630244530.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001630244530.1
AtriAmTr_v1.0_scaffold001690246620.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001690246620.1
AtriAmTr_v1.0_scaffold001690246640.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001690246640.1
AtriAmTr_v1.0_scaffold001730248070.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001730248070.1
AtriAmTr_v1.0_scaffold001810250250.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001810250250.1
AtriAmTr_v1.0_scaffold002030253830.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold002030253830.1
AtriAmTr_v1.0_scaffold002030253840.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold002030253840.1
AtriAmTr_v1.0_scaffold002030253860.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold002030253860.1
AtriAmTr_v1.0_scaffold002600256440.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold002600256440.1
AtriAmTr_v1.0_scaffold003520258010.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold003520258010.1