Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT14389 SQE4569 CAS35857

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT327781 SMO1/SMO24067 CPI11261 CYP51G1190 FK1234 HYD11364 DWF71524 DWF5888 DWF11689

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET21316 DWF4/CYP90B1290 CPD/CYP90A1103 ROT3/CYP90C1193 CYP90D1142 BR6OX1/CYP85A140 BR6OX2/CYP85A268

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (569) Bryophytes (5698) Lycophytes/Ferns (189) Gymnosperms (791) Basal angiosperms (1411) Monocots (17019) Eudicots (61245)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
actinidia_hemsleyana_GWHPCBQA010597	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA010597
actinidia_hemsleyana_GWHPCBQA010733	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA010733
actinidia_hemsleyana_GWHPCBQA011422	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA011422
actinidia_hemsleyana_GWHPCBQA012421	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA012421
actinidia_hemsleyana_GWHPCBQA012802	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA012802
actinidia_hemsleyana_GWHPCBQA013482	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA013482
actinidia_hemsleyana_GWHPCBQA013483	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA013483
actinidia_hemsleyana_GWHPCBQA013994	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA013994
actinidia_hemsleyana_GWHPCBQA015112	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA015112
actinidia_hemsleyana_GWHPCBQA015113	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA015113
actinidia_hemsleyana_GWHPCBQA015114	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA015114
actinidia_hemsleyana_GWHPCBQA015115	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA015115
actinidia_hemsleyana_GWHPCBQA015269	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA015269
actinidia_hemsleyana_GWHPCBQA015471	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA015471
actinidia_hemsleyana_GWHPCBQA015503	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA015503
actinidia_hemsleyana_GWHPCBQA015504	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA015504
actinidia_hemsleyana_GWHPCBQA015505	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA015505
actinidia_hemsleyana_GWHPCBQA016658	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016658
actinidia_hemsleyana_GWHPCBQA016659	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA016659
actinidia_hemsleyana_GWHPCBQA018133	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA018133
actinidia_hemsleyana_GWHPCBQA018464	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA018464
actinidia_hemsleyana_GWHPCBQA018483	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA018483
actinidia_hemsleyana_GWHPCBQA018895	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA018895
actinidia_hemsleyana_GWHPCBQA018896	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA018896
actinidia_hemsleyana_GWHPCBQA018897	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA018897
actinidia_hemsleyana_GWHPCBQA018898	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA018898
actinidia_hemsleyana_GWHPCBQA019627	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA019627
actinidia_hemsleyana_GWHPCBQA019660	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA019660
actinidia_hemsleyana_GWHPCBQA019676	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA019676
actinidia_hemsleyana_GWHPCBQA020298	actinidia_hemsleyana	actinidia_hemsleyana_GWHPCBQA020298