Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT13441 SQE4023 CAS46700

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT324927 SMO1/SMO26895 CPI1777 CYP51G14290 FK858 HYD1962 DWF76507 DWF5271 DWF11016

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET22338 DWF4/CYP90B12383 CPD/CYP90A12379 ROT3/CYP90C1396 CYP90D167 BR6OX1/CYP85A1120 BR6OX2/CYP85A265

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Eudicots (108415)

Current lineage filter: Eudicots. All species, family, module, and gene statistics below have been narrowed to this lineage.

Species Distribution

Chrysanthemum seticuspe

Dianthus caryophyllus

Lineage Summary

Module Gene List

Gene	Species	Protein ID
Agluscaffold32912_cov900196850	alnus_glutinosa	Agluscaffold32912_cov900196850
Agluscaffold34115_cov900203820	alnus_glutinosa	Agluscaffold34115_cov900203820
Agluscaffold3545_cov1010212580	alnus_glutinosa	Agluscaffold3545_cov1010212580
Agluscaffold3545_cov1010212640	alnus_glutinosa	Agluscaffold3545_cov1010212640
Agluscaffold36193_cov900215450	alnus_glutinosa	Agluscaffold36193_cov900215450
Agluscaffold36193_cov900215470	alnus_glutinosa	Agluscaffold36193_cov900215470
Agluscaffold36193_cov900215490	alnus_glutinosa	Agluscaffold36193_cov900215490
Agluscaffold36193_cov900215510	alnus_glutinosa	Agluscaffold36193_cov900215510
Agluscaffold36193_cov900215530	alnus_glutinosa	Agluscaffold36193_cov900215530
Agluscaffold36193_cov900215570	alnus_glutinosa	Agluscaffold36193_cov900215570
Agluscaffold37823_cov770222130	alnus_glutinosa	Agluscaffold37823_cov770222130
Agluscaffold38527_cov1060224770	alnus_glutinosa	Agluscaffold38527_cov1060224770
Agluscaffold40683_cov1050233520	alnus_glutinosa	Agluscaffold40683_cov1050233520
Agluscaffold41520_cov1040238890	alnus_glutinosa	Agluscaffold41520_cov1040238890
Agluscaffold41520_cov1040238900	alnus_glutinosa	Agluscaffold41520_cov1040238900
Agluscaffold418_cov940240970	alnus_glutinosa	Agluscaffold418_cov940240970
Agluscaffold4233_cov890242750	alnus_glutinosa	Agluscaffold4233_cov890242750
Agluscaffold424407_cov1120244540	alnus_glutinosa	Agluscaffold424407_cov1120244540
Agluscaffold424463_cov1080247570	alnus_glutinosa	Agluscaffold424463_cov1080247570
Agluscaffold424620_cov970255080	alnus_glutinosa	Agluscaffold424620_cov970255080
Agluscaffold424775_cov620261520	alnus_glutinosa	Agluscaffold424775_cov620261520
Agluscaffold424775_cov620261530	alnus_glutinosa	Agluscaffold424775_cov620261530
Agluscaffold425460_cov1020269200	alnus_glutinosa	Agluscaffold425460_cov1020269200
Agluscaffold425554_cov670271050	alnus_glutinosa	Agluscaffold425554_cov670271050
Agluscaffold425586_cov1030271380	alnus_glutinosa	Agluscaffold425586_cov1030271380
Agluscaffold425649_cov990274040	alnus_glutinosa	Agluscaffold425649_cov990274040
Agluscaffold42569_cov1020274570	alnus_glutinosa	Agluscaffold42569_cov1020274570
Agluscaffold425760_cov950274830	alnus_glutinosa	Agluscaffold425760_cov950274830
Agluscaffold425964_cov1040276290	alnus_glutinosa	Agluscaffold425964_cov1040276290
Agluscaffold425964_cov1040276300	alnus_glutinosa	Agluscaffold425964_cov1040276300