Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT13441 SQE4023 CAS46700

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT324927 SMO1/SMO26895 CPI1777 CYP51G14290 FK858 HYD1962 DWF76507 DWF5271 DWF11016

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET22338 DWF4/CYP90B12383 CPD/CYP90A12379 ROT3/CYP90C1396 CYP90D167 BR6OX1/CYP85A1120 BR6OX2/CYP85A265

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Eudicots (108415)

Current lineage filter: Eudicots. All species, family, module, and gene statistics below have been narrowed to this lineage.

Species Distribution

Chrysanthemum seticuspe

Dianthus caryophyllus

Lineage Summary

Module Gene List

Gene	Species	Protein ID
Agluscaffold427253_cov1080279010	alnus_glutinosa	Agluscaffold427253_cov1080279010
Agluscaffold43127_cov1040282820	alnus_glutinosa	Agluscaffold43127_cov1040282820
Agluscaffold4354_cov1010284410	alnus_glutinosa	Agluscaffold4354_cov1010284410
Agluscaffold4425_cov1020285980	alnus_glutinosa	Agluscaffold4425_cov1020285980
Agluscaffold45070_cov600288990	alnus_glutinosa	Agluscaffold45070_cov600288990
Agluscaffold45857_cov720293080	alnus_glutinosa	Agluscaffold45857_cov720293080
Agluscaffold46329_cov1090295440	alnus_glutinosa	Agluscaffold46329_cov1090295440
Agluscaffold46748_cov800297040	alnus_glutinosa	Agluscaffold46748_cov800297040
Agluscaffold46748_cov800297050	alnus_glutinosa	Agluscaffold46748_cov800297050
Agluscaffold4750_cov1030300270	alnus_glutinosa	Agluscaffold4750_cov1030300270
Agluscaffold4804_cov1070302470	alnus_glutinosa	Agluscaffold4804_cov1070302470
Agluscaffold50038_cov1000310960	alnus_glutinosa	Agluscaffold50038_cov1000310960
Agluscaffold50240_cov1030312210	alnus_glutinosa	Agluscaffold50240_cov1030312210
Agluscaffold50272_cov550312230	alnus_glutinosa	Agluscaffold50272_cov550312230
Agluscaffold5072_cov1050313740	alnus_glutinosa	Agluscaffold5072_cov1050313740
Agluscaffold5243_cov1030318260	alnus_glutinosa	Agluscaffold5243_cov1030318260
Agluscaffold5326_cov650320930	alnus_glutinosa	Agluscaffold5326_cov650320930
Agluscaffold5356_cov1060322630	alnus_glutinosa	Agluscaffold5356_cov1060322630
Agluscaffold5680_cov1070333520	alnus_glutinosa	Agluscaffold5680_cov1070333520
Agluscaffold5693_cov1290334290	alnus_glutinosa	Agluscaffold5693_cov1290334290
Agluscaffold5740_cov960335710	alnus_glutinosa	Agluscaffold5740_cov960335710
Agluscaffold5740_cov960335760	alnus_glutinosa	Agluscaffold5740_cov960335760
Agluscaffold5740_cov960335770	alnus_glutinosa	Agluscaffold5740_cov960335770
Agluscaffold5747_cov1020335930	alnus_glutinosa	Agluscaffold5747_cov1020335930
Agluscaffold5805_cov1070337900	alnus_glutinosa	Agluscaffold5805_cov1070337900
Agluscaffold5805_cov1070337910	alnus_glutinosa	Agluscaffold5805_cov1070337910
Agluscaffold585_cov910340340	alnus_glutinosa	Agluscaffold585_cov910340340
Agluscaffold585_cov910340360	alnus_glutinosa	Agluscaffold585_cov910340360
Agluscaffold59319_cov990341530	alnus_glutinosa	Agluscaffold59319_cov990341530
Agluscaffold59889_cov1040343460	alnus_glutinosa	Agluscaffold59889_cov1040343460