Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT13441 SQE4023 CAS46700

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT324927 SMO1/SMO26895 CPI1777 CYP51G14290 FK858 HYD1962 DWF76507 DWF5271 DWF11016

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET22338 DWF4/CYP90B12383 CPD/CYP90A12379 ROT3/CYP90C1396 CYP90D167 BR6OX1/CYP85A1120 BR6OX2/CYP85A265

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Eudicots (108415)

Current lineage filter: Eudicots. All species, family, module, and gene statistics below have been narrowed to this lineage.

Species Distribution

Chrysanthemum seticuspe

Dianthus caryophyllus

Lineage Summary

Module Gene List

Gene	Species	Protein ID
AcruScioBoG_4119_HRSCAF_41380206800.1	amaranthus_cruentus	AcruScioBoG_4119_HRSCAF_41380206800.1
AcruScioBoG_4119_HRSCAF_41380207730.1	amaranthus_cruentus	AcruScioBoG_4119_HRSCAF_41380207730.1
AcruScioBoG_4119_HRSCAF_41380209450.1	amaranthus_cruentus	AcruScioBoG_4119_HRSCAF_41380209450.1
AcruScioBoG_4119_HRSCAF_41380209450.2	amaranthus_cruentus	AcruScioBoG_4119_HRSCAF_41380209450.2
AcruScioBoG_4119_HRSCAF_41380211280.1	amaranthus_cruentus	AcruScioBoG_4119_HRSCAF_41380211280.1
AcruScioBoG_4119_HRSCAF_41380211290.1	amaranthus_cruentus	AcruScioBoG_4119_HRSCAF_41380211290.1
AcruScioBoG_52287_HRSCAF_524340217130.1	amaranthus_cruentus	AcruScioBoG_52287_HRSCAF_524340217130.1
AcruScioBoG_52287_HRSCAF_524340219060.1	amaranthus_cruentus	AcruScioBoG_52287_HRSCAF_524340219060.1
AcruScioBoG_52287_HRSCAF_524340219060.10	amaranthus_cruentus	AcruScioBoG_52287_HRSCAF_524340219060.10
AcruScioBoG_52287_HRSCAF_524340219060.11	amaranthus_cruentus	AcruScioBoG_52287_HRSCAF_524340219060.11
AcruScioBoG_52287_HRSCAF_524340219060.12	amaranthus_cruentus	AcruScioBoG_52287_HRSCAF_524340219060.12
AcruScioBoG_52287_HRSCAF_524340219060.13	amaranthus_cruentus	AcruScioBoG_52287_HRSCAF_524340219060.13
AcruScioBoG_52287_HRSCAF_524340219060.14	amaranthus_cruentus	AcruScioBoG_52287_HRSCAF_524340219060.14
AcruScioBoG_52287_HRSCAF_524340219060.2	amaranthus_cruentus	AcruScioBoG_52287_HRSCAF_524340219060.2
AcruScioBoG_52287_HRSCAF_524340219060.3	amaranthus_cruentus	AcruScioBoG_52287_HRSCAF_524340219060.3
AcruScioBoG_52287_HRSCAF_524340219060.4	amaranthus_cruentus	AcruScioBoG_52287_HRSCAF_524340219060.4
AcruScioBoG_52287_HRSCAF_524340219060.5	amaranthus_cruentus	AcruScioBoG_52287_HRSCAF_524340219060.5
AcruScioBoG_52287_HRSCAF_524340219060.6	amaranthus_cruentus	AcruScioBoG_52287_HRSCAF_524340219060.6
AcruScioBoG_52287_HRSCAF_524340219060.7	amaranthus_cruentus	AcruScioBoG_52287_HRSCAF_524340219060.7
AcruScioBoG_52287_HRSCAF_524340219060.8	amaranthus_cruentus	AcruScioBoG_52287_HRSCAF_524340219060.8
AcruScioBoG_52287_HRSCAF_524340219060.9	amaranthus_cruentus	AcruScioBoG_52287_HRSCAF_524340219060.9
AcruScioBoG_8578_HRSCAF_86170226560.1	amaranthus_cruentus	AcruScioBoG_8578_HRSCAF_86170226560.1
AcruScioBoG_8578_HRSCAF_86170226790.2	amaranthus_cruentus	AcruScioBoG_8578_HRSCAF_86170226790.2
AcruScioBoG_94932_HRSCAF_951340228100.1	amaranthus_cruentus	AcruScioBoG_94932_HRSCAF_951340228100.1
AcruScioBoG_94932_HRSCAF_951340228100.2	amaranthus_cruentus	AcruScioBoG_94932_HRSCAF_951340228100.2
AcruScioBoG_94932_HRSCAF_951340228100.3	amaranthus_cruentus	AcruScioBoG_94932_HRSCAF_951340228100.3
AcruScioBoG_94932_HRSCAF_951340228990.1	amaranthus_cruentus	AcruScioBoG_94932_HRSCAF_951340228990.1
AcruScioBoG_94932_HRSCAF_951340228990.2	amaranthus_cruentus	AcruScioBoG_94932_HRSCAF_951340228990.2
AcruScioBoG_94932_HRSCAF_951340231330.1	amaranthus_cruentus	AcruScioBoG_94932_HRSCAF_951340231330.1
AcruScioBoG_94932_HRSCAF_951340231330.2	amaranthus_cruentus	AcruScioBoG_94932_HRSCAF_951340231330.2