Rosmarinic acid portable pathway

Rosmarinic acid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Rosmarinic Acid Reaction Route

Phenylalanine donor branch plus tyrosine acceptor branch. This pathway merges two aromatic amino-acid branches; after RAS condensation, CYP98A-type hydroxylases form rosmarinic acid with CPR electron-transfer support. The page displays annotatable members in the current reference library.

7 curated steps/modules

Phenylalanine Donor Branch

Substrate

L-Phenylalanine

C₉H₁₁NO₂

C₆H₅-CH₂-CH(NH₂)-COOH

Deamination into the phenylpropanoid donor branch

PAL7015

Product

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

PAL converts L-phenylalanine to cinnamate as the entry step toward the 4-coumaroyl-CoA donor.

Substrate

trans-Cinnamic acid

C₉H₈O₂

C₆H₅-CH=CH-COOH

Cinnamate 4-hydroxylation

C4H48992

Product

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

C4H/CYP73A forms 4-coumaric acid for downstream CoA activation.

Substrate

4-Coumaric acid

C₉H₈O₃

4-HO-C₆H₄-CH=CH-COOH

Substrate

CoA

C₂₁H₃₆N₇O₁₆P₃S

CoA-SH

CoA ligation to form the RAS acyl donor

4CL25914

Product

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

4CL generates 4-coumaroyl-CoA as the acyl donor for the RAS condensation reaction.

Tyrosine Acceptor Branch And Condensation

Substrate

L-Tyrosine

C₉H₁₁NO₃

4-HO-C₆H₄-CH₂-CH(NH₂)-COOH

Transamination to 4-hydroxyphenylpyruvate

TAT14219

Product

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

TAT routes L-tyrosine into the 4-hydroxyphenylpyruvate acceptor branch.

Substrate

4-Hydroxyphenylpyruvate

C₉H₈O₄

4-HO-C₆H₄-CH₂-C(=O)-COOH

Reduction to the HPLA acceptor

HPPR5065

Product

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

HPPR reduces 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate.

Substrate

4-Coumaroyl-CoA

C₃₀H₃₈N₇O₁₈P₃S

4-HO-C₆H₄-CH=CH-C(=O)-SCoA

Substrate

4-Hydroxyphenyllactate

C₉H₁₀O₄

4-HO-C₆H₄-CH₂-CH(OH)-COOH

Ester-forming condensation

RAS13433

Product

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

4-HO-C₆H₄-CH=CH-C(=O)-O-CH(COOH)-CH₂-Ar-OH

RAS couples the acyl donor with the HPLA acceptor to form the direct rosmarinic-acid precursor.

Substrate

4-Coumaroyl-4'-hydroxyphenyllactate

C₁₈H₁₆O₇

mono-catechol ester precursor

CYP98A14-type hydroxylation with CPR electron-transfer support

CPR5870

Product

Rosmarinic acid

C₁₈H₁₆O₈

caffeoyl-O-3,4-dihydroxyphenyllactate

CYP98A14 or close CYP98A enzymes catalyse 3/3' hydroxylation; CPR in the current reference library represents electron-transfer support for this P450 reaction and should not be interpreted as CPR alone performing hydroxylation.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (388) Bryophytes (9418) Lycophytes/Ferns (423) Gymnosperms (1285) Basal angiosperms (2118) Monocots (23710) Eudicots (83094)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
aconitum_vilmorinianum_GWHPBRAL020987	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL020987
aconitum_vilmorinianum_GWHPBRAL021495	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021495
aconitum_vilmorinianum_GWHPBRAL021513	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021513
aconitum_vilmorinianum_GWHPBRAL021716	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021716
aconitum_vilmorinianum_GWHPBRAL021717	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021717
aconitum_vilmorinianum_GWHPBRAL021718	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021718
aconitum_vilmorinianum_GWHPBRAL021719	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021719
aconitum_vilmorinianum_GWHPBRAL021721	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021721
aconitum_vilmorinianum_GWHPBRAL021723	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021723
aconitum_vilmorinianum_GWHPBRAL021724	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021724
aconitum_vilmorinianum_GWHPBRAL021725	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021725
aconitum_vilmorinianum_GWHPBRAL021727	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021727
aconitum_vilmorinianum_GWHPBRAL021728	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021728
aconitum_vilmorinianum_GWHPBRAL021729	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021729
aconitum_vilmorinianum_GWHPBRAL021731	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021731
aconitum_vilmorinianum_GWHPBRAL021732	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021732
aconitum_vilmorinianum_GWHPBRAL021734	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021734
aconitum_vilmorinianum_GWHPBRAL021736	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021736
aconitum_vilmorinianum_GWHPBRAL021738	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021738
aconitum_vilmorinianum_GWHPBRAL021740	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021740
aconitum_vilmorinianum_GWHPBRAL021742	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021742
aconitum_vilmorinianum_GWHPBRAL021743	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021743
aconitum_vilmorinianum_GWHPBRAL021745	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021745
aconitum_vilmorinianum_GWHPBRAL021748	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021748
aconitum_vilmorinianum_GWHPBRAL021750	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021750
aconitum_vilmorinianum_GWHPBRAL021755	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021755
aconitum_vilmorinianum_GWHPBRAL021789	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021789
aconitum_vilmorinianum_GWHPBRAL022112	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL022112
aconitum_vilmorinianum_GWHPBRAL022122	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL022122
aconitum_vilmorinianum_GWHPBRAL022476	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL022476