Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT14389 SQE4569 CAS35857

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT327781 SMO1/SMO24067 CPI11261 CYP51G1190 FK1234 HYD11364 DWF71524 DWF5888 DWF11689

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET21316 DWF4/CYP90B1290 CPD/CYP90A1103 ROT3/CYP90C1193 CYP90D1142 BR6OX1/CYP85A140 BR6OX2/CYP85A268

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (569) Bryophytes (5698) Lycophytes/Ferns (189) Gymnosperms (791) Basal angiosperms (1411) Monocots (17019) Eudicots (61245)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
AtriAmTr_v1.0_scaffold000370108010.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000370108010.1
AtriAmTr_v1.0_scaffold000380108640.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000380108640.1
AtriAmTr_v1.0_scaffold000380108660.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000380108660.1
AtriAmTr_v1.0_scaffold000380108720.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000380108720.1
AtriAmTr_v1.0_scaffold000410116390.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000410116390.1
AtriAmTr_v1.0_scaffold000410116460.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000410116460.1
AtriAmTr_v1.0_scaffold000430120150.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000430120150.1
AtriAmTr_v1.0_scaffold000430120160.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000430120160.1
AtriAmTr_v1.0_scaffold000430120210.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000430120210.1
AtriAmTr_v1.0_scaffold000440120780.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000440120780.1
AtriAmTr_v1.0_scaffold000600154290.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000600154290.1
AtriAmTr_v1.0_scaffold000620159830.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000620159830.1
AtriAmTr_v1.0_scaffold000650162290.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000650162290.1
AtriAmTr_v1.0_scaffold000660165710.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000660165710.1
AtriAmTr_v1.0_scaffold000660165930.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000660165930.1
AtriAmTr_v1.0_scaffold000660165980.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000660165980.1
AtriAmTr_v1.0_scaffold000710174800.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000710174800.1
AtriAmTr_v1.0_scaffold000710175640.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000710175640.1
AtriAmTr_v1.0_scaffold000790187230.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000790187230.1
AtriAmTr_v1.0_scaffold000840190430.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000840190430.1
AtriAmTr_v1.0_scaffold000890196020.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000890196020.1
AtriAmTr_v1.0_scaffold000890196030.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000890196030.1
AtriAmTr_v1.0_scaffold000890196040.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000890196040.1
AtriAmTr_v1.0_scaffold000890196050.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000890196050.1
AtriAmTr_v1.0_scaffold000890196080.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000890196080.1
AtriAmTr_v1.0_scaffold000890196200.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000890196200.1
AtriAmTr_v1.0_scaffold000890196210.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold000890196210.1
AtriAmTr_v1.0_scaffold001030208900.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001030208900.1
AtriAmTr_v1.0_scaffold001030209200.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001030209200.1
AtriAmTr_v1.0_scaffold001030209210.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001030209210.1