Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT14389 SQE4569 CAS35857

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT327781 SMO1/SMO24067 CPI11261 CYP51G1190 FK1234 HYD11364 DWF71524 DWF5888 DWF11689

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET21316 DWF4/CYP90B1290 CPD/CYP90A1103 ROT3/CYP90C1193 CYP90D1142 BR6OX1/CYP85A140 BR6OX2/CYP85A268

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (569) Bryophytes (5698) Lycophytes/Ferns (189) Gymnosperms (791) Basal angiosperms (1411) Monocots (17019) Eudicots (61245)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
AtriAmTr_v1.0_scaffold001030209220.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001030209220.1
AtriAmTr_v1.0_scaffold001170220550.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001170220550.1
AtriAmTr_v1.0_scaffold001190221680.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001190221680.1
AtriAmTr_v1.0_scaffold001330229780.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001330229780.1
AtriAmTr_v1.0_scaffold001400233440.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001400233440.1
AtriAmTr_v1.0_scaffold001400233450.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001400233450.1
AtriAmTr_v1.0_scaffold001530240220.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001530240220.1
AtriAmTr_v1.0_scaffold001540240630.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001540240630.1
AtriAmTr_v1.0_scaffold001560242230.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001560242230.1
AtriAmTr_v1.0_scaffold001630244530.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001630244530.1
AtriAmTr_v1.0_scaffold001690246620.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001690246620.1
AtriAmTr_v1.0_scaffold001690246640.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001690246640.1
AtriAmTr_v1.0_scaffold001730248070.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001730248070.1
AtriAmTr_v1.0_scaffold001810250250.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold001810250250.1
AtriAmTr_v1.0_scaffold002030253830.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold002030253830.1
AtriAmTr_v1.0_scaffold002030253840.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold002030253840.1
AtriAmTr_v1.0_scaffold002030253860.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold002030253860.1
AtriAmTr_v1.0_scaffold002600256440.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold002600256440.1
AtriAmTr_v1.0_scaffold003520258010.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold003520258010.1
AtriAmTr_v1.0_scaffold010100260940.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold010100260940.1
AtriAmTr_v1.0_scaffold027640264290.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold027640264290.1
AtriAmTr_v1.0_scaffold029860264620.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold029860264620.1
AtriAmTr_v1.0_scaffold051970267700.1	amborella_trichopoda	AtriAmTr_v1.0_scaffold051970267700.1
AnanContig000100002500.1	ammopiptanthus_nanus	AnanContig000100002500.1
AnanContig000100002530.1	ammopiptanthus_nanus	AnanContig000100002530.1
AnanContig000100002540.1	ammopiptanthus_nanus	AnanContig000100002540.1
AnanContig000100004490.1	ammopiptanthus_nanus	AnanContig000100004490.1
AnanContig000260012790.1	ammopiptanthus_nanus	AnanContig000260012790.1
AnanContig000260012920.1	ammopiptanthus_nanus	AnanContig000260012920.1
AnanContig000260015880.1	ammopiptanthus_nanus	AnanContig000260015880.1