Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT14389 SQE4569 CAS35857

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT327781 SMO1/SMO24067 CPI11261 CYP51G1190 FK1234 HYD11364 DWF71524 DWF5888 DWF11689

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET21316 DWF4/CYP90B1290 CPD/CYP90A1103 ROT3/CYP90C1193 CYP90D1142 BR6OX1/CYP85A140 BR6OX2/CYP85A268

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (569) Bryophytes (5698) Lycophytes/Ferns (189) Gymnosperms (791) Basal angiosperms (1411) Monocots (17019) Eudicots (61245)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
AnanContig010530339910.1	ammopiptanthus_nanus	AnanContig010530339910.1
AnanContig010530341890.1	ammopiptanthus_nanus	AnanContig010530341890.1
AnanContig010550344930.1	ammopiptanthus_nanus	AnanContig010550344930.1
AnanContig010990370680.1	ammopiptanthus_nanus	AnanContig010990370680.1
AedgHiC_scaffold_10002010	amphicarpaea_edgeworthii	AedgHiC_scaffold_10002010
AedgHiC_scaffold_100037580	amphicarpaea_edgeworthii	AedgHiC_scaffold_100037580
AedgHiC_scaffold_100041350	amphicarpaea_edgeworthii	AedgHiC_scaffold_100041350
AedgHiC_scaffold_100041810	amphicarpaea_edgeworthii	AedgHiC_scaffold_100041810
AedgHiC_scaffold_100042950	amphicarpaea_edgeworthii	AedgHiC_scaffold_100042950
AedgHiC_scaffold_100042960	amphicarpaea_edgeworthii	AedgHiC_scaffold_100042960
AedgHiC_scaffold_100044750	amphicarpaea_edgeworthii	AedgHiC_scaffold_100044750
AedgHiC_scaffold_100044770	amphicarpaea_edgeworthii	AedgHiC_scaffold_100044770
AedgHiC_scaffold_10004580	amphicarpaea_edgeworthii	AedgHiC_scaffold_10004580
AedgHiC_scaffold_10004640	amphicarpaea_edgeworthii	AedgHiC_scaffold_10004640
AedgHiC_scaffold_100046880	amphicarpaea_edgeworthii	AedgHiC_scaffold_100046880
AedgHiC_scaffold_100046950	amphicarpaea_edgeworthii	AedgHiC_scaffold_100046950
AedgHiC_scaffold_100046970	amphicarpaea_edgeworthii	AedgHiC_scaffold_100046970
AedgHiC_scaffold_100047690	amphicarpaea_edgeworthii	AedgHiC_scaffold_100047690
AedgHiC_scaffold_100047720	amphicarpaea_edgeworthii	AedgHiC_scaffold_100047720
AedgHiC_scaffold_100048410	amphicarpaea_edgeworthii	AedgHiC_scaffold_100048410
AedgHiC_scaffold_100048420	amphicarpaea_edgeworthii	AedgHiC_scaffold_100048420
AedgHiC_scaffold_100048450	amphicarpaea_edgeworthii	AedgHiC_scaffold_100048450
AedgHiC_scaffold_100051580	amphicarpaea_edgeworthii	AedgHiC_scaffold_100051580
AedgHiC_scaffold_100053000	amphicarpaea_edgeworthii	AedgHiC_scaffold_100053000
AedgHiC_scaffold_100053770	amphicarpaea_edgeworthii	AedgHiC_scaffold_100053770
AedgHiC_scaffold_10010970	amphicarpaea_edgeworthii	AedgHiC_scaffold_10010970
AedgHiC_scaffold_10012810	amphicarpaea_edgeworthii	AedgHiC_scaffold_10012810
AedgHiC_scaffold_10012910	amphicarpaea_edgeworthii	AedgHiC_scaffold_10012910
AedgHiC_scaffold_10023330	amphicarpaea_edgeworthii	AedgHiC_scaffold_10023330
AedgHiC_scaffold_10023610	amphicarpaea_edgeworthii	AedgHiC_scaffold_10023610