Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT14389 SQE4569 CAS35857

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT327781 SMO1/SMO24067 CPI11261 CYP51G1190 FK1234 HYD11364 DWF71524 DWF5888 DWF11689

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET21316 DWF4/CYP90B1290 CPD/CYP90A1103 ROT3/CYP90C1193 CYP90D1142 BR6OX1/CYP85A140 BR6OX2/CYP85A268

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (569) Bryophytes (5698) Lycophytes/Ferns (189) Gymnosperms (791) Basal angiosperms (1411) Monocots (17019) Eudicots (61245)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
aconitum_vilmorinianum_GWHPBRAL003519	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL003519
aconitum_vilmorinianum_GWHPBRAL003520	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL003520
aconitum_vilmorinianum_GWHPBRAL003522	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL003522
aconitum_vilmorinianum_GWHPBRAL003797	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL003797
aconitum_vilmorinianum_GWHPBRAL004018	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL004018
aconitum_vilmorinianum_GWHPBRAL004491	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL004491
aconitum_vilmorinianum_GWHPBRAL004556	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL004556
aconitum_vilmorinianum_GWHPBRAL004850	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL004850
aconitum_vilmorinianum_GWHPBRAL004853	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL004853
aconitum_vilmorinianum_GWHPBRAL005227	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL005227
aconitum_vilmorinianum_GWHPBRAL005229	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL005229
aconitum_vilmorinianum_GWHPBRAL005861	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL005861
aconitum_vilmorinianum_GWHPBRAL006257	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL006257
aconitum_vilmorinianum_GWHPBRAL006427	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL006427
aconitum_vilmorinianum_GWHPBRAL006429	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL006429
aconitum_vilmorinianum_GWHPBRAL006430	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL006430
aconitum_vilmorinianum_GWHPBRAL006633	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL006633
aconitum_vilmorinianum_GWHPBRAL006637	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL006637
aconitum_vilmorinianum_GWHPBRAL006817	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL006817
aconitum_vilmorinianum_GWHPBRAL007556	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL007556
aconitum_vilmorinianum_GWHPBRAL007653	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL007653
aconitum_vilmorinianum_GWHPBRAL007936	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL007936
aconitum_vilmorinianum_GWHPBRAL008203	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL008203
aconitum_vilmorinianum_GWHPBRAL008602	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL008602
aconitum_vilmorinianum_GWHPBRAL009583	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL009583
aconitum_vilmorinianum_GWHPBRAL009705	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL009705
aconitum_vilmorinianum_GWHPBRAL009906	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL009906
aconitum_vilmorinianum_GWHPBRAL010877	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL010877
aconitum_vilmorinianum_GWHPBRAL010886	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL010886
aconitum_vilmorinianum_GWHPBRAL011196	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL011196