Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT14389 SQE4569 CAS35857

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT327781 SMO1/SMO24067 CPI11261 CYP51G1190 FK1234 HYD11364 DWF71524 DWF5888 DWF11689

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET21316 DWF4/CYP90B1290 CPD/CYP90A1103 ROT3/CYP90C1193 CYP90D1142 BR6OX1/CYP85A140 BR6OX2/CYP85A268

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (569) Bryophytes (5698) Lycophytes/Ferns (189) Gymnosperms (791) Basal angiosperms (1411) Monocots (17019) Eudicots (61245)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
aconitum_vilmorinianum_GWHPBRAL011539	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL011539
aconitum_vilmorinianum_GWHPBRAL011603	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL011603
aconitum_vilmorinianum_GWHPBRAL011859	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL011859
aconitum_vilmorinianum_GWHPBRAL012177	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL012177
aconitum_vilmorinianum_GWHPBRAL012192	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL012192
aconitum_vilmorinianum_GWHPBRAL012195	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL012195
aconitum_vilmorinianum_GWHPBRAL012367	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL012367
aconitum_vilmorinianum_GWHPBRAL012410	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL012410
aconitum_vilmorinianum_GWHPBRAL012771	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL012771
aconitum_vilmorinianum_GWHPBRAL012782	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL012782
aconitum_vilmorinianum_GWHPBRAL012783	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL012783
aconitum_vilmorinianum_GWHPBRAL012875	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL012875
aconitum_vilmorinianum_GWHPBRAL013500	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL013500
aconitum_vilmorinianum_GWHPBRAL013510	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL013510
aconitum_vilmorinianum_GWHPBRAL013511	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL013511
aconitum_vilmorinianum_GWHPBRAL013817	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL013817
aconitum_vilmorinianum_GWHPBRAL014036	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL014036
aconitum_vilmorinianum_GWHPBRAL014045	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL014045
aconitum_vilmorinianum_GWHPBRAL014635	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL014635
aconitum_vilmorinianum_GWHPBRAL014713	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL014713
aconitum_vilmorinianum_GWHPBRAL014715	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL014715
aconitum_vilmorinianum_GWHPBRAL014948	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL014948
aconitum_vilmorinianum_GWHPBRAL014950	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL014950
aconitum_vilmorinianum_GWHPBRAL015040	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL015040
aconitum_vilmorinianum_GWHPBRAL015314	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL015314
aconitum_vilmorinianum_GWHPBRAL015323	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL015323
aconitum_vilmorinianum_GWHPBRAL015328	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL015328
aconitum_vilmorinianum_GWHPBRAL015329	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL015329
aconitum_vilmorinianum_GWHPBRAL015538	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL015538
aconitum_vilmorinianum_GWHPBRAL015743	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL015743