Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT14389 SQE4569 CAS35857

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT327781 SMO1/SMO24067 CPI11261 CYP51G1190 FK1234 HYD11364 DWF71524 DWF5888 DWF11689

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET21316 DWF4/CYP90B1290 CPD/CYP90A1103 ROT3/CYP90C1193 CYP90D1142 BR6OX1/CYP85A140 BR6OX2/CYP85A268

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (569) Bryophytes (5698) Lycophytes/Ferns (189) Gymnosperms (791) Basal angiosperms (1411) Monocots (17019) Eudicots (61245)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
aconitum_vilmorinianum_GWHPBRAL015788	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL015788
aconitum_vilmorinianum_GWHPBRAL016244	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL016244
aconitum_vilmorinianum_GWHPBRAL016524	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL016524
aconitum_vilmorinianum_GWHPBRAL016525	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL016525
aconitum_vilmorinianum_GWHPBRAL016876	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL016876
aconitum_vilmorinianum_GWHPBRAL017320	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL017320
aconitum_vilmorinianum_GWHPBRAL017394	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL017394
aconitum_vilmorinianum_GWHPBRAL017401	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL017401
aconitum_vilmorinianum_GWHPBRAL018142	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL018142
aconitum_vilmorinianum_GWHPBRAL018195	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL018195
aconitum_vilmorinianum_GWHPBRAL018371	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL018371
aconitum_vilmorinianum_GWHPBRAL018373	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL018373
aconitum_vilmorinianum_GWHPBRAL018737	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL018737
aconitum_vilmorinianum_GWHPBRAL018781	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL018781
aconitum_vilmorinianum_GWHPBRAL018962	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL018962
aconitum_vilmorinianum_GWHPBRAL019010	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL019010
aconitum_vilmorinianum_GWHPBRAL019011	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL019011
aconitum_vilmorinianum_GWHPBRAL019015	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL019015
aconitum_vilmorinianum_GWHPBRAL019020	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL019020
aconitum_vilmorinianum_GWHPBRAL019971	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL019971
aconitum_vilmorinianum_GWHPBRAL019985	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL019985
aconitum_vilmorinianum_GWHPBRAL020494	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL020494
aconitum_vilmorinianum_GWHPBRAL020640	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL020640
aconitum_vilmorinianum_GWHPBRAL020641	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL020641
aconitum_vilmorinianum_GWHPBRAL020665	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL020665
aconitum_vilmorinianum_GWHPBRAL021390	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021390
aconitum_vilmorinianum_GWHPBRAL021717	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL021717
aconitum_vilmorinianum_GWHPBRAL022441	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL022441
aconitum_vilmorinianum_GWHPBRAL022561	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL022561
aconitum_vilmorinianum_GWHPBRAL022996	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL022996