Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT14389 SQE4569 CAS35857

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT327781 SMO1/SMO24067 CPI11261 CYP51G1190 FK1234 HYD11364 DWF71524 DWF5888 DWF11689

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET21316 DWF4/CYP90B1290 CPD/CYP90A1103 ROT3/CYP90C1193 CYP90D1142 BR6OX1/CYP85A140 BR6OX2/CYP85A268

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (569) Bryophytes (5698) Lycophytes/Ferns (189) Gymnosperms (791) Basal angiosperms (1411) Monocots (17019) Eudicots (61245)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
aconitum_vilmorinianum_GWHPBRAL023125	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL023125
aconitum_vilmorinianum_GWHPBRAL023278	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL023278
aconitum_vilmorinianum_GWHPBRAL024066	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL024066
aconitum_vilmorinianum_GWHPBRAL024814	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL024814
aconitum_vilmorinianum_GWHPBRAL025127	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL025127
aconitum_vilmorinianum_GWHPBRAL025133	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL025133
aconitum_vilmorinianum_GWHPBRAL025581	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL025581
aconitum_vilmorinianum_GWHPBRAL025649	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL025649
aconitum_vilmorinianum_GWHPBRAL025699	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL025699
aconitum_vilmorinianum_GWHPBRAL025702	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL025702
aconitum_vilmorinianum_GWHPBRAL025704	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL025704
aconitum_vilmorinianum_GWHPBRAL025718	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL025718
aconitum_vilmorinianum_GWHPBRAL025733	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL025733
aconitum_vilmorinianum_GWHPBRAL025734	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL025734
aconitum_vilmorinianum_GWHPBRAL025735	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL025735
aconitum_vilmorinianum_GWHPBRAL025736	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL025736
aconitum_vilmorinianum_GWHPBRAL026937	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL026937
aconitum_vilmorinianum_GWHPBRAL027233	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL027233
aconitum_vilmorinianum_GWHPBRAL027314	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL027314
aconitum_vilmorinianum_GWHPBRAL027659	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL027659
aconitum_vilmorinianum_GWHPBRAL027663	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL027663
aconitum_vilmorinianum_GWHPBRAL027665	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL027665
aconitum_vilmorinianum_GWHPBRAL027667	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL027667
aconitum_vilmorinianum_GWHPBRAL027669	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL027669
aconitum_vilmorinianum_GWHPBRAL027677	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL027677
aconitum_vilmorinianum_GWHPBRAL027785	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL027785
aconitum_vilmorinianum_GWHPBRAL028530	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL028530
aconitum_vilmorinianum_GWHPBRAL028614	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL028614
aconitum_vilmorinianum_GWHPBRAL029993	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL029993
aconitum_vilmorinianum_GWHPBRAL030074	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL030074