Steroid portable pathway

Steroid pathway definition imported from the 2026-05-17 portable BLAST/HMM package.

Representative Steroid Reaction Route

Squalene to phytosterols and brassinosteroid branch. The steroid route starts from triterpenoid precursors, proceeds through squalene, 2,3-oxidosqualene, and cycloartenol, and then branches toward phytosterols and brassinosteroids; long branches are shown as curated modules.

3 curated steps/modules

Sterol Scaffold Formation

Substrate

Farnesyl diphosphate

C₁₅H₂₈O₇P₂

C₁₅ prenyl-OPP

Squalene synthesis, epoxidation, and cyclization

SQS/FDFT14389 SQE4569 CAS35857

Product

Cycloartenol

C₃₀H₅₀O

tetracyclic sterol nucleus with cyclopropyl side

SQS/SQE/CAS convert FPP-derived linear triterpenes into the plant sterol core.

Substrate

Cycloartenol-series intermediates

C₂₈-C₃₀ sterols

tetracyclic sterol nucleus

Demethylation, reduction, and side-chain remodeling

SMT1/SMT2/SMT327781 SMO1/SMO24067 CPI11261 CYP51G1190 FK1234 HYD11364 DWF71524 DWF5888 DWF11689

Product

Phytosterols

C₂₈H₄₈O / C₂₉H₅₀O

sterol nucleus-C₈/C₉ side chain

This segment converts cycloartenol-series intermediates into phytosterol pools such as sitosterol and campesterol.

Brassinosteroid Branch

Substrate

Campesterol-like sterols

C₂₈H₄₈O

sterol nucleus-C₈ side chain

Sterol hydroxylation/oxidation to brassinosteroids

DET21316 DWF4/CYP90B1290 CPD/CYP90A1103 ROT3/CYP90C1193 CYP90D1142 BR6OX1/CYP85A140 BR6OX2/CYP85A268

Product

Brassinosteroids

C₂₈ oxygenated lactones

polyhydroxylated steroid lactone

DET2 and CYP90/CYP85 enzymes route campesterol-like substrates toward brassinosteroid signaling molecules.

Lineage Distribution

Assignments under this module are distributed across the following plant lineages.

All Lineages Algae (569) Bryophytes (5698) Lycophytes/Ferns (189) Gymnosperms (791) Basal angiosperms (1411) Monocots (17019) Eudicots (61245)

Species Distribution

Lineage Summary

Module Gene List

Gene	Species	Protein ID
aconitum_vilmorinianum_GWHPBRAL030877	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL030877
aconitum_vilmorinianum_GWHPBRAL031332	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL031332
aconitum_vilmorinianum_GWHPBRAL031749	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL031749
aconitum_vilmorinianum_GWHPBRAL031894	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL031894
aconitum_vilmorinianum_GWHPBRAL032195	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL032195
aconitum_vilmorinianum_GWHPBRAL033227	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL033227
aconitum_vilmorinianum_GWHPBRAL033499	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL033499
aconitum_vilmorinianum_GWHPBRAL033500	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL033500
aconitum_vilmorinianum_GWHPBRAL033503	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL033503
aconitum_vilmorinianum_GWHPBRAL033504	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL033504
aconitum_vilmorinianum_GWHPBRAL034096	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL034096
aconitum_vilmorinianum_GWHPBRAL034533	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL034533
aconitum_vilmorinianum_GWHPBRAL034534	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL034534
aconitum_vilmorinianum_GWHPBRAL034535	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL034535
aconitum_vilmorinianum_GWHPBRAL034622	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL034622
aconitum_vilmorinianum_GWHPBRAL034735	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL034735
aconitum_vilmorinianum_GWHPBRAL035028	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL035028
aconitum_vilmorinianum_GWHPBRAL035187	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL035187
aconitum_vilmorinianum_GWHPBRAL035191	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL035191
aconitum_vilmorinianum_GWHPBRAL035194	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL035194
aconitum_vilmorinianum_GWHPBRAL035276	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL035276
aconitum_vilmorinianum_GWHPBRAL035277	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL035277
aconitum_vilmorinianum_GWHPBRAL035278	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL035278
aconitum_vilmorinianum_GWHPBRAL037770	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL037770
aconitum_vilmorinianum_GWHPBRAL039298	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL039298
aconitum_vilmorinianum_GWHPBRAL043418	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL043418
aconitum_vilmorinianum_GWHPBRAL046048	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL046048
aconitum_vilmorinianum_GWHPBRAL047791	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL047791
aconitum_vilmorinianum_GWHPBRAL049261	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL049261
aconitum_vilmorinianum_GWHPBRAL049462	aconitum_vilmorinianum	aconitum_vilmorinianum_GWHPBRAL049462